摘要
研究沙冬青细胞经茉莉酸甲酯(MeJA)瞬时处理后发生的Ca2+离子流、H2O2含量及质膜电位的变化情况。结果表明:MeJA引起沙冬青细胞H+质子内流、H2O2积累及膜电位去极化。胞内钙库抑制剂钌红预处理细胞后,抑制了MeJA处理引起的H+质子内流、H2O2的积累及膜电位去极化;表明MeJA瞬时处理后,胞内钙库中Ca2+的释放是细胞随后产生的响应。H2O2清除剂预处理彻底抑制MeJA引起的H+质子内流及质膜电位的去极化反应,证明H2O2的积累位于H+质子内流的上游,H+质子内流是沙冬青细胞膜电位去极化的主要原因。
This study investigated changes in Ca2＋ flux,hydrogen peroxide content and membrane potential of Ammopiptanthus mongolicus cells in response to methyl jasmonate treatment.Results showed that methyl jasmonate caused proton influx,hydrogen peroxide production and membrane depolarization,which were completely inhibited by pretreatment with intracellular calcium store release inhibitor ruthenium red,demonstrating that calcium release from intracellular calcium store was necessary for proton influx,hydrogen peroxide production and membrane depolarization.Both proton influx and membrane depolarization,triggered by treatment with methyl jasmonate,were inhibited by pretreatment with a hydrogen peroxide scavenger,indicating that hydrogen peroxide production was also necessary for proton influx which was the main reason of membrane depolarization.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第10期24-29,共6页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(30871727)
长江学者与创新团队发展计划项目(PCSIRT0607)
关键词
茉莉酸甲酯
沙冬青
Ca2+离子流
H2O2
膜电位
methyl jasmonate
Ammopiptanthus mongolicus
Ca2＋ ion flux
hydrogen peroxide
membrane potential
