摘要
目的:探讨低频率电刺激对GABA介导的背根神经节神经元膜电位去极化反应的影响。方法:新鲜分离的大鼠DRG坐骨神经干标本,通过细胞内微电极记录技术,应用波宽5 ms,强度2 V,频率分别为2 Hz和10 Hz的脉冲波作用于大鼠坐骨神经远端,观察刺激前后GABA(3×10-4mol/L)介导的DRG神经元膜电位去极化反应幅值的变化。结果:低频电刺激(2 Hz1、0 Hz)对GABA介导的DRG神经元膜电位去极化反应均有抑制作用,10 Hz时抑制作用达到最大,反应幅值从17.00±3.39mV降至12.00±2.45mV(P<0.05)。刺激频率增加,电刺激对GABA介导DRG神经元膜去极化反应抑制作用持续时间也逐渐延长,10 Hz时作用持续时间达到最长(>72 min)。结论:电刺激可抑制DRG神经元膜GABA去极化反应,从而易化伤害性信息的传入,导致疼痛。
Objective： To investigate the effect of low-frequencies electrical stimulation on GABA- induced depolarization on rat dorsal root ganglion neurons. Methods： We dissociated the DRG attached dorsal roots and sciatic nerve. With intracellular microelectrode recording methods, low frequencies electrical stimulation （5 ms,2 V, frequencies are 2 Hz and 10 Hz） were applied to neurons freshly dissociated from rat sciatic nerve. We observed the variation of membrane potential amplitude of GABA （3 × 10^-4 mol/l,） - induced depolarization in rat dorsal root ganglion neurons before and after electrical stimulation. Results： Each frequency of electrical stimulation （2 Hz and 10 Hz） can itdlibit GABA - induced membrane potential in dorsal root ganglion neurons.The maximum inhibitory effect was happened when the frequencies of stimulation was 10 Hz, the value of depolarising response decreased from 17.00 ±3.39mV to 12.00 ± 2.45mV （ P 〈 0.05 ）. As frequency of stimulation strengthened, the duration of recovery from depressant effect of GABA - induced depolarization in dorsal root ganglion neurons also gradually prolonged. Recovery time was longer （ 〉 72 min） when the frequencies of stimulation was 10 Hz. Conclusion： electrical stimulation canmake nocipetive information transmit easily through inhibit GABA- induced depolarization in rat dorsal root ganglion neurons, which leads to the pain.
出处
《农垦医学》
2008年第5期353-356,共4页
Journal of Nongken Medicine
基金
国家自然科学基金资助项目
301600262
石河子大学高层次人才科研启动基金项目
RCZX200546
关键词
电刺激
DRG
去极化反应
疼痛
electrical stimulation
dorsal root ganglion （DRG）
depolarize reaction
pain
