摘要
目的：建立除湿止痒软膏中蛇床子素的含量测定方法。方法：采用高效液相色谱法,色谱柱为Agilent TC-C18柱（250mm×4.6mm,5μm）,以乙腈∶水（65∶35）为流动相,流速为1mL.min-1,检测波长为322nm。结果：蛇床子素在0.1282-0.7692μg范围内与峰面积呈良好的线性关系,m=0.0005S＋0.0009（r=1）;平均回收率为100.00%,RSD为0.15%,n=6。结论：该方法简便、可靠、准确,专属性强,可用于该制剂的质量控制。
Objective ： To establish a method for determination of osthole in Chunshizhiyang ointment. Methods：Samples were analyzed on an Agilent TC--C18 column （2250mm×4.6mm,5μm）using acetonitrile-water （ 65 ： 35 ） as mobile phase. Speed of flow- was 1.0mL· min^-1 and examination wave length was 322nm. Results： Osthole showed a good linearity in the range of 0.1282-0.7692 μ g, regression equation was m=0.0005S＋0.0009 （r=l）and tile average recovery was 100.00% （RSD=0.15%, n=6 ）. Conclusion ： The established methods are simple, accurate, reliable and can be used for the quality control of Chushizhiyang ointment.
出处
《辽宁中医药大学学报》
CAS
2012年第12期189-190,共2页
Journal of Liaoning University of Traditional Chinese Medicine
