摘要
合成与结构表征了 4个钴卟啉配合物 Co(Por) ,Por=5 ,10 ,15 ,2 0 -四 - [(3,4,5 -三甲氧基苯基 )卟啉(1) ,(对甲基苯基 )卟啉 (2 ) ,(对氯苯基 )卟啉 (3) ,(对磺酸基苯基 )卟啉 (4) ].并用它们作为超氧化物歧化酶 (SOD)和过氧化氢酶 (CAT)模型物进行了双功能模拟酶活性的测试 :核黄素 -蛋氨酸光照法证实 ,在 10 - 5～ 10 - 6 mol/ L 浓度范围内 4种配合物均有消除超氧自由基 (O-·2 )的作用 ;分光光度法证实具有催化 H2 O2 的分解作用 ,其分解率随浓度的增大而增大 ;用小鼠肝匀浆法测定 ,四种钴卟啉均表明有良好的抗脂质过氧化作用 .
The four cobalt porphyrins [Co(3,4,5\|MeO\|TPP)(1); Co(TTP)(2); Co(4\|Cl\|TPP)(3); Co(4\|HSO 3\|TPP)(4)] were synthesized and considered as two mimic of superoxide dismutaes(SOD) and catalase (CAT). The catalytic efficiency of them on deplete the harmful radical have been proved using riboflavin\|methionine photoreduction method in the concentration range of 10 -6 ~10 -5 mol/L. The catalytic efficiency of them at decomposing H 2O 2 has been performed by spectrophotometer. The percentage of decomposing H 2O 2 while the increasing the concentration of the imitating compounds. The lipid peroxidation produced in liver homogenated of mice was determined by spectrophotometer detecting the content of MDA.
出处
《武汉大学学报（自然科学版）》
CSCD
2000年第2期150-152,共3页
Journal of Wuhan University(Natural Science Edition)
基金
国家自然科学基金!资助项目 ( 2 9972 0 3 5 )
湖北省自然科学基金资助项目!( 98J0 43 )
关键词
钴卟啉
合成
SOD
氧自由基
毒性
CAT
消除作用
cobalt porphyrin
synthesis
superoxide dismutaes
catalase
two functional mimic of enzyme
