摘要
以四-（对-磺酸基苯基）钴卟啉[Co－TPPS4]作为超氧化物歧化酶（SOD）和过氧化氢酶（CAT）双功能模拟酶，研究其仿SOD和CAT活性及抗脂质过氧化活性。结果应用核黄素-蛋氨酸光照法证实其在10－5～10-6mol／L浓度范围内有清除超氧阴离子自由基的作用；分光光度法证实其能催化过氧化氢的分解，当其浓度由5μmol／L增至30μmol／L时，分解率由11．9％增至62．4％；小鼠肝匀浆法测定Co－TPPS4、SOD、CAT，均表明具有较好的抗脂质过氧化的作用。
In this article,the tetraphenylporphinesulfonatocobalt (Co-TPPS4) was used as dual function mimicry of superoxide dismutase (SOD) and catalase (CAT). The first function, scavenging O2, has been proved by using riboflavin-methionine photoreduction method in the concentration range of 10-5～10-6 mol/ L. The second function, scavenging H2O2, has been demonstrated by means of spectrophotometry. When the concentration of Co-TPPS4 increased from 5 μmol/L to 30 μmol/L, the decomposing rate of H2O2 increased from 11.9% to 62. 4%. Co-TPPS4, SOD and CAT were measured by the liver homogenate technique of mice and the results showed that they had obvious action of decreasing the lipid peroxidation.
出处
《同济医科大学学报》
CSCD
1997年第5期344-347,共4页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金!No.39370794
