摘要
目的探讨hMLH1在直肠上皮内瘤变与早期直肠癌组织中的表达情况及其早期诊断价值。方法采用PV-9000二步法免疫组织化学检测技术对术后确诊的28例早期浸润性直肠癌、36例直肠上皮内瘤变和30例正常直肠黏膜组织的石蜡切片进行hMLHl蛋白表达的检测。结果正常直肠黏膜组织、直肠上皮内瘤变组织和早期浸润性直肠癌组织hMLH1蛋白的阳性表达率分别为100％（30／30）、77．8％（28／36）和39．3％（11／28），3组比较差异有统计学意义（P〈0．05）。直肠上皮内瘤变组织hMLH1的阳性表达与患者的年龄、性别、肿瘤最大径、异型增生、肿瘤类型和距肛缘的距离均无关（P〉0．05）；早期直肠癌组织hMLH1阳性表达与其分化程度有关（P〈0．05）。结论hMLHI基因表达缺失可能是直肠癌发生的早期事件之一,hMLH1蛋白检测对于上皮内瘤变和早期直肠癌中两种疾病的早期诊断有一定的临床价值。
Objective To explore whether the abnormality of hMLH1 gene may be an early event of carcinogenesis in rectal carcinoma, and to evaluate the diagnostic value in differentiation between intraepithelial neoplasm and early stage of colorectal carcinoma. Methods The expression of hMLH1 protein in 28 cases with early invasive rectal carcinoma （EIRC）, 36 cases with rectal intraepithelial neoplasm （RIEN）, and 30 cases with normal rectal mucosa （NRM） which were collected through surgical operations were detected by PV-9000 immunohistochemical method. Results The positive expression rates of hMLH1 protein were 100% （30/30）, 77.8% （28/36）, and 39.3% （11/28） in NRM, RIEN, and EIRC respectively. The difference was statistically significant between RIEN and EIRC （P=0.002）, and the difference was also statistically significant between RIEN and NRM （P= 0.006）. The positive expression of hMLH1 was not related to age, gender, tumor maximum diameter, dysplasia, tumor types, and distance from the anal verge in RIEV group （P〉0.05）. In EIRC group, hMLH1 was associated with tumor differentiation （P〈0.05）. Conclusion hMLH1 gene deletion may be an early event during carcinogenesis of rectal carcinoma, which may be useful in differentiation of intraepithelial neoplasm from early rectal carcinoma.
出处
《中华胃肠外科杂志》
CAS
2012年第11期1162-1165,共4页
Chinese Journal of Gastrointestinal Surgery
基金
民政部十一五老年医学科研项目（47-2-17）
