摘要
目的探讨人类错配修复基因hMLH1、hMSH2及p53在肺癌组织中的表达及意义。方法运用免疫组织化学SP法对56例肺癌组织中hMLH1、hMSH2及p53的表达进行检测。结果56例肺癌组织中hMLH1的阳性表达率为35%,hMSH2阳性表达率为28.6%,分化程度高者阳性率显著高于分化程度低者(P<0.01),有淋巴结转移者hMLH1及hMSH2阳性率低于无淋巴结转移者(P<0.05),不同病理组织学类型之间hMLH1及hMSH2表达无显著差别(P>0.05);56例肺癌组织中,p53阳性率为51.8%,分化程度低者p53阳性率高于分化程度高者(P<0.05),有淋巴结转移者p53阳性率高于无淋巴结转移者(P<0.01),不同病理组织学类型之间p53阳性率无差别(P>0.05);hMLH1及hMSH2阴性表达者的p53阳性率高于hMLH1及hMSH2阳性表达者(P<0.05)。结论hMLH1及hMSH2基因的缺陷及p53的表达与肺癌的发生发展过程并与分化程度及有否淋巴结转移有关。
Objective To investigate the expression of human mismatch repair gene（hMLH1/hMSH2） and p53 in lung cancer patients and its significance. Methods Expression of hMLH1/hMSH2 and p53 in 56 lung cancer patients was determined by S - P immunohistochemieal staining. Results The positive expression rates of hMLH1 and hMSH2 in 56 lung cancer eases were 35% and 28.6% respectively. The positive rates of expression of hMLH1/hMSH2 in the patients at high and moderate differentiation stages were significantly higher than those of the others （ P 〈 0.01 ）. Meanwhile, the positive rate of eases with lymph node metastasis was lower than that of the patients without lymph node metastasis （ P 〈 0.05）. It wasn＇t positively correlated with pathological types. The positive expression rate of p53 in 56 lung cancer eases was 51.8% and in the patients at high and moderate differentiation stages were lower than those of others （P 〈 0.01）. The rate in patients with lymph node metastasis was significantly higher than that of patients without metastasis （P 〈 0.05）. The rate of p53 in hMLH1/hMSH2 positive lung cancer tissue was higher than that in hMLH1/ hMSH2 negative tissue （ P 〈 0.05）. Conclusion The abnormal expression of hMLH1/hMSH2 and p53 might be involved in the carcinogenesis of lung cancer and correlated with differentiation stages and lymph node metastasis.
出处
《中国热带医学》
CAS
2007年第4期521-523,共3页
China Tropical Medicine
