摘要
为获得理想的L-乳酸产生菌,选择适合根霉属微生物生长的土样,利用溴甲酚绿平板结合摇瓶复筛的方法得到了一株有一定L-乳酸积累能力的米根霉Rhizopus oryzae CS323。摇瓶发酵试验显示,在未优化发酵条件的情况下发酵48h,米根霉CS323L-乳酸积累量达到50.1g/L,是一株有良好改造潜力的L-乳酸产生菌,适合作为进一步诱变育种的出发菌株。
In order to obtain an ideal strain producing L-lactic acid, Rhizopus oryzae strain CS323 was obtained by bromocresol green agar plate and shaking flask screening from soil samples. Shaking flask test showed that, without optimization of fermentation conditions, L-lactic acid accumulation of the strain CS323 was 50. lg/L after 48h fermentation. It was a potential L-lactic acid producer suitable for further mutation.
出处
《菌物学报》
CAS
CSCD
北大核心
2012年第6期956-962,共7页
Mycosystema
基金
湖南省教育厅资助项目(No.06C090)
晓庄学院微生物分子生物学湖南省重点实验室开放基金(No.MM0802)
关键词
米根霉
溴甲酚绿平板
摇瓶发酵试验
Rhizopus oryzae, bromoeresol green agar, shaking flask test
