摘要
目的构建恶性疟原虫海南分离株（FCC1／HN）裂殖子表面蛋白MSA2的真核表达质粒pcDNA3／MSA2，为疟疾核酸疫苗及蛋白疫苗的研制奠定基础。方法采用PCR技术对FCC1／HN基因组DNAMSA2基因进行扩增，扩增产物经纯化后用BamHI和EcoRI双酶切，然后走向克隆入真核表达质粒pcDNA3，连接产物转化大肠杆菌TG1，再用相同的内切酸酶切和PCR扩增对重组子进行鉴定。结果筛选出的重组子为编码PCC1／HCMSA2基因片段的重组质粒pcDNA3／MSA2。结论编码FCC1／HNMSA2基因片段真该表达质粒pcDNA3／MSA2的构建，为疟疾核酸疫苗及蛋白疫苗的研制奠定基础。
Using polymersase chain reaction (PCR) technique, the MSA2 gene was amplified from genomic DNA of southem China isolate (FCC1/HN) of Plasmodium falciparum.The PCR product was purified and digested with BamHI and EcoRI,and the generated DNA fragment was cloned into plasmid pcDNA3,and transformed into Escherichia coli (E.coli)strain TG1.The recombinant plasmids were screened and identified by BamHI and EcoRI digestion and PCR amplification.The result demonstrated that the recombinant plasmid pcDNA3/MSA2 gene from FCC1/HN isolate of Plsmodium falciparum.These findings are very important for the expression of MSA2 in uitro and the development of malaria vaccine.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第2期29-31,共3页
Chinese Journal of Zoonoses
