摘要
目的 探讨光动力作用 (PDT)对体外培养的人胰腺癌细胞的杀伤效应。 方法 以 2株人胰腺癌细胞系P3和SW1990为研究对象 ,采用血卟啉衍生物 (HPD)作为光敏剂 ,用高压钠灯 (功率密度 2 5mW /cm2 )为光源 ,以系列浓度的HPD经不同剂量的光照后 ,用MTT法测定PDT对胰腺癌细胞的相对抑制率。 结果 随光敏剂浓度的升高和照光剂量的增加 ,光动力作用对细胞的相对抑制率逐渐增大 ,在低光动力剂量下明显上升 ,随后上升渐趋缓慢达平台期 ;相同光动力剂量下 ,PDT对 2株细胞的相对抑制率存在明显差异 (P <0 0 1) ,LD90剂量条件亦不同。 结论 光动力作用对体外培养的人胰腺癌细胞具有明确的杀伤效应 ,其对细胞的相对抑制率具有显著的剂量效应关系 ;
Objective To observe the effect of photodynamic therapy(PDT)on human pancreatic carcinoma cell lines in vitro. Methods Two lines of human pancreatic carcinoma cell P3 and SW1990 were studied to investigate the killing role of PDT with hematoporphyrin derivative(HPD) as photosensitizer and sodium lamp as light source. The cultured cells were treated with serial concentrations of HPD followed by irradiation of different dosages of visible light, then MTT colorometric assay was applied to measure the relative inhibitory rate of PDT for the cells. Results The relative inhibitory rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of light. Under the low photodynamic dose, it increased rapidly, and gradually slowed down to reach the plateau. Under the same photodynamic dose, significant difference in the inhibitory rate between the two lines was observed (P<0 01), and the LD90 was also different. Conclusions PDT has a significant killing effect on human pancreatic carcinoma cell lines in vitro, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and light irradiation. The sensitivity of the two cell lines to PDT is different.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2000年第3期204-207,共4页
Chinese Journal of Surgery
基金
高等院校博士学科点专项科研基金
"九五"国家科技攻关基金! (969070305)
关键词
血卟啉衍生物
光化学疗法
胰腺肿瘤
Hematopor phyrin derivative
Photochemotherapy
Pancreatic neoplasms
