摘要
目的:研究乳腺癌细胞中IL-2、IL-4表达与化疗药物敏感性的关系。方法:选取病理诊断为乳腺癌(术前未行放疗、化疗)的手术切除标本20例,体外分离细胞作培养,应用四甲基偶氮唑盐(MTT)法分析表柔比星(EADM)、5-氟脲嘧啶(5-Fu)、盖诺(NVB)、顺铂(DDP)4种化疗药物对乳腺癌肿瘤细胞的相对抑制率,通过免疫组织化学染色法分析乳腺癌组织IL-2、IL-4的表达。结果:0.1×PPC浓度时乳腺癌细胞对4种化疗药物的敏感率分别为EADM30%、5-Fu20%、NVB45%、DDP25%;IL-2(+)的乳腺癌细胞对4种化疗药物敏感性均高于IL-2(-)的乳腺癌细胞(P<0.05),IL-2阳性表达程度与乳腺癌细胞对化疗药物的敏感性存在正相关关系(P<0.05)。IL-4(+)的乳腺癌细胞对EADM、NVB敏感性均低于IL-4(-)的乳腺癌细胞(P<0.05),IL-4阳性表达程度与乳腺癌细胞对化EADM、NVB的敏感性存在负相关关系(P<0.05)。结论:IL-2、IL-4的表达与乳腺癌细胞对化疗药物敏感性有关,IL-2、IL-4可作为乳腺癌临床化疗疗效预测的2个指标。对IL-2、IL-4表达的联合检测可以对乳腺癌化疗药物的选择起到指导作用。
Objective：To investigate the relationship of the expression of IL-2 and IL-4 in breast cancer cells with chemosensitivity. Methods：Surgical samples of breast cancer,diagnosed by pathology,were obtained from The First Affiliated Hospital of Chongqing Medical University. The cancer sample cells were cultured separately in the incubator at 37℃ ,5%CO2 in vitro. The rate of inhibition of cancer cells by 4 kindsof anticancer drugs,which were EADM,5-Fu,NVB and DDP,were assayed by MTT method. Immunohistochemistry was used to detect the expression of IL-2 and IL-4 in the breast cancer tissues. Results ：Sensitivity rates of 20 breast cancer cells in 0.1 × PPC within 48h in vitro were 30% （EADM）, 20% （5-Fu）, 45%（NVB）and 25%（DDP）.Respectively the rate of inhibition of EADM,5-Fu,NVB and DDP were significantly higher in the IL-2 positive cancer cells than in the IL-2 negative cancer cells. A positive correlation was found between expression of IL-2 and chemosensitivity for all the 4 anticancer drugs. The rates of inhibition of EADM and NVB were significantly lower in the IL-4 positive cancer cells than in the IL-4 negative cancer cells. A negative correlation was found between expression of IL-4 and chemosensitivity for the 2 anticancer drugs. Conclusions：The expression of IL-2 and IL-4 are related with the chemosensitivity of the breast cancer cells,and they may become one of the markers for judging the effect of chemotherapy in clinic. The combined detection of IL-2 and IL-4 may be taken as an index of the selection of anticancer drugs.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第7期835-838,共4页
Journal of Chongqing Medical University
