摘要
目的 :建立恶性肿瘤细胞经血循环向骨骼肌运行的动物模型 ,观察骨骼肌转移瘤罕见性现象 ,并进一步探讨骨骼肌微血管内皮细胞粘附分子 ( VCAM- 1)的意义。方法 :Wistar大鼠 60只 ,随机分成实验组与对照组。实验组经髂动脉注入 Walker2 5 6癌肉瘤单细胞悬液 ( 10 4) ,对照组经尾静脉注入同一瘤细胞 ( 10 6) ,两组分别于 2h、 1d、 7d、 14 d各处死 6只 ,余下 6只自然死亡 ,观察比较肺内、大腿骨骼肌内肿瘤形成情况及微血管内皮细胞VCAM- 1表达情况。结果 :实验组注入瘤细胞后 ,第 7天在大腿骨骼肌微血管周围见瘤细胞团 ( 3只 ) ,肌组织内无转移灶形成 ,部分鼠见骨骼肌旁软组织内有转移灶形成 ;对照组注入瘤细胞后肺内 7d形成肿瘤灶 ,14 d及自然死亡者 10 0 %形成广泛肺转移灶 (共 17只 ) ,两组比较差异有显著意义 ( P<0 .0 0 1)。实验组大腿骨骼肌微血管内皮VCAM- 1阳性率在注瘤 d7以后显著上升 ,且实验组大腿骨骼肌与对照组肺微血管内皮细胞 VCAM- 1阳性率无显著差异 ( P=0 .5 )。结论 :临床上广泛存在的恶性肿瘤骨骼肌转移罕见性 ,可在动物模型中观察到。骨骼肌微血管内皮细胞 VCAM- 1的表达 。
Objective:To establish an animal model for blood borne metastasis of malignant tumors, and to evaluate the significance of vascular cell adhesion molecule (VCAM 1) in the rarity of metastases in skeletal muscles. Methods:60 Wistar rats were chosen and randomly divided into two paired groups. 30 rats in the experimental group were injected with tumor cells (Walker256, 10 4) through iliac artery, and paired rats in the control group were injected with the same tumor cells (10 6, considering the damages in circulation) through tail veins. The animals in each group were sacrificed in batches (6rats each) 2 hours、 1 day、 7 days、 14 days after the start of the procedures, or died naturally (6rats). The femur muscles and lungs were observed grossly and histiologically, the VCAM 1 expressions in the microvessels in these organs were estimated meanwhile. Results:In the experimental group, tumor cells near the blood vessels in the femur muscles were rarely seen in rats (3rats in all) sacrificed 7 days and 14 days after the procedures, there was no definite metastase within parenchymal skeletal muscles but there are some metastases in soft tissues beside the skeletal muscles; In the control group, metastases began to form in lungs 7 days after tail veins were injected with tumor cells, and were seen in the lungs of all the rats that were sacrificed 14 days later or died naturally (17rats in all) ( P <0 001). In the experimental group, an significant higher positive rate of VCAM 1 in femur muscles were seen 7 days after iliac arteries were injected with tumor cells and thereafter ( P <0 001). There was no significant difference between the positive rate of VCAM 1 in femur muscles in the experimental group and that in the lungs in the control group ( P =0 5). Conclusion:The rarity of metastases in skeletal muscles, generally known in clinic, can appear in animal models. How ever, this rarity is not related to VCAM 1 expressions in microvessels in skeletal muscles.
出处
《军医进修学院学报》
2000年第1期8-10,共3页
Academic Journal of Pla Postgraduate Medical School
