摘要
目的研究骨骼肌微环境因素对恶性肿瘤细胞增殖的影响,评价其在骨骼肌转移瘤罕见性中的意义。方法原代培养新生Wistar大鼠骨骼肌细胞,用MTT法分析、比较其条件性上清液(MMCM)对恶性肿瘤细胞(小鼠骨髓瘤细胞株SP2/0、鼻咽癌细胞株CNE、肺巨细胞癌细胞株PLA801C、人食管鳞癌细胞株Eca109、宫颈癌细胞株Hela)及正常非肿瘤细胞(金黄地鼠肾细胞株BHK-21)的体外抑瘤作用。结果MMCM分别与SP2/0、CNE、PLA801C、Eca109、Hela及BHK-21细胞共同培养后,对5株恶性肿瘤细胞的增殖均有明显抑制作用,而正常非肿瘤细胞株则未受影响。结论新生大鼠骨骼肌细胞可产生选择性抑制恶性肿瘤细胞增殖的抑瘤物质,这种抑瘤物质可能是临床上骨骼肌内罕见转移瘤现象的生物学基础。
Objective To explore the organic microenvironmental effect of skeletal muscles on the proliferation of malignant ceils and evaluate its significance in the rarity of metastases in skeletal muscles. Methods Prepare the primary culture of new born Wistar rat skeletal muscle ceils and collect the culture supernatant as murine skeletal muscle conditioned medium（MMCM）. Analyze the suppressive effect in vitro of MMCM on the proliferation of malignant tumor ceils including murine myeloma ceil strain SP2/0, nasopharyngeal carcinoma ceil strain CNE, pulmonary cytomegalic carcinoma ceil strain PLA-801C , human oesophageal squamous ceil carci- noma strain Eca109 and cervical carcinoma ceil strain Hela. Results MMCM showed significant suppressive effect on the proliferations of all the five tumor cell strains. However, it showed no significant effect on normal cells. Conclusion The tumor inhibitors derived from new born Wistar rat skeletal muscle ceils selectively suppressed the proliferation of malignant tumors and provided a biological basis for the study on rare metastases in skeletal muscles.
出处
《中国生物制品学杂志》
CAS
CSCD
2006年第3期262-264,共3页
Chinese Journal of Biologicals
基金
全军"十.五"医药卫生科研基金资助项目(01MB073).
关键词
骨骼肌
抑瘤物
抑制作用
Skeletal muscle
Tumor inhibitor
Suppressive effect
