摘要
目的:观察人参皂苷Rg1诱导人神经干细胞(neural stem cells of human,hNSCs)的功能表达。方法:采用全细胞膜片钳技术分析人参皂苷Rg1诱导hNSCs分化7 d时,神经元样细胞的膜电生理特性以及钠、钾离子通道的功能表达。结果:人参皂苷Rg1(20 mg·L-1)诱导hNSCs分化7 d时,神经元样细胞的膜静息电位为(-45.70±2.63)mV,膜电容为(26.89±1.91)pF,膜输入阻抗为(877.51±20.44)MΩ(与对照组相比均P<0.05);电压依赖性的快速激活、快速失活的内向Na+电流,检出率50%,平均峰值为(711.48±158.03)pA(与对照组相比P<0.05);外向K+电流的主要成分是快速激活快速失活的瞬时外向K+电流和延迟整流外向K+电流,其平均峰值为(1 070.42±177.18)pA(与对照组相比P<0.05)。结论:人参皂苷Rg1可以促进hNSCs功能表达和成熟。
Objective： To observe the effects of ginsenoside Rg1 on the functional expression of human neural stem cells （hNSCs）. Method： The membrane electrophysiological properties and sodium and potassium ion channels in the hNSCs induced by Rg1 were analyzed using the whole-cell patch-clamp. Result： On the 7th day, the neuron-like cells derived from ginsenoside Rg1 （20 mg · L ^- 1 ） -induced NSCs show ： （1） The resting membrane potential ： （ - 45.70 ± 2.63 ） mV, the membrane capacitance ： （ 26.89 ±1.91 ） pF, the membrane input impedance ： （ 877.51 ± 20.44） MΩ （ P 〈 0.05 compared with the control group, respectively） ; （2） The detection rate of inward sodium current which is rapidly activated and inactivated in voltage-dependence was 50%, and its average peak value was （711.48 ± 158.03 ） pA （P 〈 0.05 compared with the control group） ; （3）The outward potassium currents were composed of rapidly activated and inactivated transient outward potassium current and delayed rectifier outward potassium current, and its average peak value was （1 070.42 ± 177.18） pA（P 〈0.05 compared with the control group）. Conclusion： Ginsenoside Rg1 can promote the fnnctional expression and maturity of hNSCs.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第22期3477-3480,共4页
China Journal of Chinese Materia Medica
基金
重庆市渝中区科技计划项目(20110310)
关键词
人参皂苷RG1
人神经干细胞
功能表达
膜片钳
ginsenoside RgI
human neural stem cell
functional expression
patch clamp
