摘要
该研究通过观察黄芪多糖对老年大鼠学习记忆的影响,试探究其改善学习记忆能力的机制。实验以自然衰老雌性SD大鼠为动物模型,随机分为老年对照组、老年黄芪多糖低、高剂量组(50,150 mg·kg-1)和老年脑复康组(阳性对照,560 mg·kg-1),各组大鼠连续灌胃给药60 d,另设青年对照组。采用旷场和Morris水迷宫测试大鼠的学习记忆能力,蛋白免疫印迹法观察海马组织相关神经可塑性蛋白N-甲基-D-天门冬氨酸受体(NMDA受体)、钙/钙调蛋白依赖的蛋白激酶Ⅱ(CaMKⅡ)、蛋白激酶A(PKA)、cAMP反应元件结合蛋白(CREB)的磷酸化水平及脑源性神经营养调控因子(BDNF)的蛋白表达水平。研究发现与青年对照组比较,老年大鼠学习和记忆能力均显著下降(P<0.01),海马神经可塑性蛋白表达明显降低(P<0.01);与老年对照组比较,黄芪多糖能明显改善老年大鼠的学习记忆损伤,上调老年大鼠海马区神经可塑性相关蛋白表达水平。结果表明黄芪多糖可能通过改善神经可塑性相关蛋白水平的异常表达,进而改善老年大鼠学习记忆能力。
To observe the effect of Astragali Radix polysaccharides （APS） on the learning and memory functions of aged rats, in order to explore its mechanism for improving the learning and memory functions. Natural aging female SD rats were selected in the animal model and randomly divided into the control group, the APS low-dose group （50 mg·kg^-1）, the APS high-dose group （150 mg·kg^-1） and the piracetam-treated group （560 mg·kg^-1）. They were orally administered with the corresponding drugs for consecutively 60 days. Besides, a young control group was set. The learning and memory functions of the rats were tested by the open-field test and the Morris water maze task. The Western-blot method was used to observe the levels of relevant neural plasticity protein N-methyl -D-aspartate receptor （NMDA receptor） in hippocampus, calcium/calmodulin dependent protein kinaseⅡ（CaMKⅡ）, protein kinase （PKA）, the phosphorylation level of CAMP response element binding protein （CREB） and the protein expression of brain derived neurotrophic factor（BDNF）. In this study, the authors found that the learning and memory functions and the hippocampus neural plasticity protein expression of the aged rat group were much lower than that of the young control group （P〈0.01）. Compared with the aged rat group, the APS group showed the significant improvement in the impaired learning and memory functions of aged rats and the up-regulation in the hippocampus neural plasticity protein expression. The results showed that APS may improve the learning and memory functions of aged rats by increasing the expressions of relevant neural plasticity proteins.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第11期2071-2075,共5页
China Journal of Chinese Materia Medica
基金
中国科学院知识创新方向性项目(KSCX2-YW-R-254)
浙江省中西医结合老年医学重点学科项目
关键词
老年大鼠
黄芪多糖
神经可塑性
aged rat
Astragali Radix polysaccharide
neural plasticity
