摘要
目的检测胃癌患者血清白细胞介素2(IL-2)和Fas配体(FasL)的表达,探讨其与胃癌临床及病理指标的关系。方法42例胃癌患者血清标本,其中男性26例,女性16例;年龄35～80岁,平均年龄60.0岁。同期正常对照者血清标本40例,其中男性25例,女性15例;年龄36～78岁,平均年龄58.8岁。应用酶联免疫吸附分析(ELISA)检测胃癌患者和正常对照者血清标本中IL-2和FasL的表达,作对比分析。同时采用单因素方差分析和Spearman相关分析探寻血清IL-2和FasL的表达与胃癌病理类型、病理分期及TNM分期的关系。统计分析采用SPSS16.0软件,P<0.05为差异有统计学意义。结果胃癌患者血清IL-2的表达显著低于正常对照者[(211.3±119.0)ng/mL vs(287.9±79.9)ng/mL;P=0.001];胃癌患者血清FasL的表达显著高于正常对照者[(2.7±1.0)ng/mL vs(1.9±1.2)ng/mL;P=0.001]。Spearman相关分析显示胃癌患者血清IL-2与FasL之间无显著相关关系。结论血清IL-2和FasL表达与胃癌的发病密切相关,可作为协同判断胃癌发病的指标。
Objective To detect the expression of interleukin 2（IL-2） and Fas ligand（FasL）, and investigate their relations with the clinical and pathological indices of gastric carcinoma. Methods A total of 42 serum samples of gastric carcinoma patient were enrolled, male 26, female 16, aged 35 - 80 years old, mean age 60.0 years old. A total of 40 serum samples of normal persons were enrolled as control, male 25, female 15, aged 36 - 78 years old, mean age 58.8 years old. The expression of serum IL-2 and FasL from 42 patients with gastric carcinoma were detected with enzyme-linked immunosorbent assay（ELISA）, and compared with the serum samples from the 40 healthy controls. The one-way ANOVA and Spearman correlation were used to investigate the relationship between the expressions of IL-2 and FasL in pathological types, pathological stage and tumor node metastasis 0＇NM） stage. The statistical analysis was performed with SPSS 16.0 software and P 〈 0.05 was considered statistically significant. Results The expression of IL-2 was significantly lower in the patients with gastric carcinoma than that of the healthy controls[（211.3 ± 119.0） ng/mL vs （287.9± 79.9） ng/mL; P = 0.001]; The expression of FasL was significantly higher in the patients with gastric carcinoma than that of healthy controls[（2.7 ± 1.0） ng/mL vs （1.9 ±1.2） ng/mL; P=0.001]. Spearman correlation showed no significant relationship between IL-2 and FasL Conclusion It is demonstrated that the expression of IL-2 and FasL which could be used as collaborative indicators, are closely related to the development of gastric carcinoma.
出处
《生物医学工程与临床》
CAS
2012年第6期592-595,共4页
Biomedical Engineering and Clinical Medicine
基金
上海市卢湾区学科带头人基金(LWX0902)
