摘要
目的探讨辛伐他汀对支气管哮喘（简称哮喘）模型大鼠血清中白介素4（interleukin-4，IL-4）和干扰素-γ（interferon-γ，IFN-γ）含量变化的影响，为辛伐他汀治疗哮喘提供实验依据。方法选取SD雄性大鼠32只，随机分为4组：正常对照组、哮喘模型组、地塞米松治疗组和辛伐他汀治疗组。每组各8只，采用卵白蛋白（ovalbumin，0VA）致敏和雾化吸人激发制备大鼠哮喘模型，用双位点夹心酶联免疫吸附试验测定各组大鼠血清中IL-4、IFN-γ含量。结果哮喘模型组血清中IFNγ含量为（83．71±8．89）ng／L明显低于正常对照组；1174含量为（106．62±13．33）ng／L，明显高于正常对照组，差异有统计学意义（P〈0．01）。与哮喘模型组相比，辛伐他汀组和地塞米松组血清中IFN-γ含量分别为（95．93±11．74）ng／L和（96．34±11．64）ng／L，明显高于哮喘模型组，差异有统计学意义（P〈0．05），而两个治疗药物组之间差异无统计学意义（P〉0．05）。与哮喘模型组相比，两个治疗药物组的IL-4含量分别为（79．08±7．34）ng／L和（73．39±7．76）ng／L，均有明显降低，差异有统计学意义（P〈O．01），但两个治疗药物组间差异无统计学意义（P〉0．05）。结论辛伐他汀和地塞米松均能显著提高哮喘大鼠血清IFN-γ的含量，降低血清IL-4含量。
Objective To study the impact of simvastatin on the asthma model rats serum interleukin-4（IL-4） and interferon-γ（IFN-γ） level changes, which provide the experimental basis for simvastatin treatment of bronchial asthma. Methods 32 male SD rats were randomly divided into four groups：normal control group, asthmatic model group, the dexamethasone treatment group and the simvastatin group,with 8 rats in each group, using ovalbumin （OVA） sensitization and aerosol inhalation to stimulate the rat model of asthma. Double sites sandwich enzyme-linked immunosorbent assay in serum IL-4 and IFN-γ levels. Results The levels of serum IFN-γ were significantly lower than the normal control group, IL-4 content was significantly higher than the normal control group, has a very significant difference （ P〈0.01）, respectively （83.71 ±8.89） ng/L and （106.62 ± 13.33） ng/L; IFN-γ in serum compared with asthmatic model group, sirnvastatin groupand dexamethasone group were （95.93±11.74） ng/L and （96.34 ± 11.64） ng/L, significantly higher than the asthma model group, a significant difference （P 〈0.05）, and no significant difference （P 〉 0.05） between the two treatment groups; compared with asthmatic model group, IL-4 levels in both treatment groups were significantly lower （ P 〈0.01） of IL-4 levels were （79.08 ± 7.34） ng/L and （73.39 ±7.76） ng/L in no significant difference between therapy groups （ P 〉0.05）. Conclusions Simvastatin and dexam-ethasone can significantly improve the level of serum IFN-γ in asthmatic rats, reducing the level of serum IL-4 had better control of asthma.
出处
《国际呼吸杂志》
2012年第20期1541-1544,共4页
International Journal of Respiration
