摘要
用分光光度法研究了利福平(Rifampicin)与7,7,8,8-四氰基对二次甲基苯醌(TCNQ)之间的荷移反应。实验表明,利福平与TCNQ间的反应在丙酮介质中进行,形成的络合物在744nm和844nm有两个吸收峰,表观摩尔吸光系数分别是2.38×104L·mol-1·cm-1和4.18×104L·mol-1·cm-1;药物浓度在1.65—57.6mg·L-1范围内服从比耳定律。用摩尔比法和等摩尔连续变化法测得荷移络合物中利福平与配体的摩尔比为1:1。用所研究方法测定了药物中利福平的含量结果令人满意,相对标准偏差为0.57%(n=5),回收率在99.0%—99.4%之间。
The charge transfer 8-tetracyan＇oquinodimethane （TCNQ） was interaction between rifampicin and 7, 7, 8, investigated by spectrophotometry. The reaction of rifampicin and TCNQ occurred in acetone medium, and the produced complex had two absorption peaks in 744nm and 844nm, and the apparent molar absorptivities were respectively 2. 38 ×10^4 L · mol-1 · cm-1 and 4.18 ×10^4L · mo1-1 · cm-1. Beers law was obeyed in the concentration range of 1.65--57.6mg ; L-;. The mole ratio of rifampiein and 7, 7, 8, 8-tetracyanoquinodimethane in complex is 1 ： 1 by mole ratio method and the equimolar continuous variation method. This proposed method was applied to the determination of rifampicin in tablets with satisfactory results. The relative standard deviation of the method was 0. 57% （n=5）. The recoveries were from 99.0% to 99.4%.
出处
《光谱实验室》
CAS
CSCD
2012年第6期3522-3524,共3页
Chinese Journal of Spectroscopy Laboratory
基金
山东省自然科学基金(Y2008B36)
