摘要
目的建立茜素红荷移分光光度法测定阿奇霉素片溶出度的方法.方法采用中国药典附录XC第三法,以250mlpH5的磷酸盐缓冲液为溶剂,转速为100r/min,经45min取样,茜素红荷移分光光度法在538nm处测定阿奇霉素的溶出度,限度为标示量的75%.结果阿奇霉素在51～255μg/ml范围内线性关系良好(r=0.9996),平均回收率为99.2%.结论该方法准确、简便,可用于阿奇霉素片溶出度的测定.
Objection To establish a method for the determination of azithromycin tablets dissolution based on charge-transfer reaction with alizarin red. Methods The dissolution test were conducted according to Pharmacopoeia of People＇s Republic of China, using phosphate buffer solution as medium, with a stirring speed of 100r/min. The solution was withdrawn after 45min. The absorbance of dissolution solutions was measured at 538nm. Dissolution limit is 75% of the labeled amount. Result Good linear correlation was achieved at the range of 50-250μg/ml azithromycin （r=0. 9996）. The mean recovery was 99.0%. Conclusion The method is accurate, simple, and suitable to the determination of azithromycin tablets dissolution.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2005年第9期533-534,567,共3页
Chinese Journal of Antibiotics
关键词
阿奇霉素片
溶出度
茜素红
荷移反应
分光光度法
Azithromycin tablets
Dissolution
Alizarin red
Charge-transfer reaction
Spectrophotometry
