摘要
乙烯响应元件结合因子(Ethylene-responsive element-binding factor,ERF)是植物中最大的转录因子家族之一。本研究以棉花叶片cDNA文库为基础,从陆地棉中棉所10号中克隆得到一个新的ERF基因,命名为GhERF8(GenBank:JN656957)。该基因编码265个氨基酸,蛋白序列中包含一个AP2保守结构域。采用荧光定量PCR(RT-PCR)的方法,对GhERF8基因在棉株不同生育时期叶片和不同组织中的表达水平进行了定量分析。结果表明,GhERF8基因在各组织中均有表达,但在成熟后期的叶片中表达量最高。GhERF8表达量与叶片衰老过程中叶绿素含量出现相反的变化趋势,推测GhERF8可能与叶片衰老有一定关系。在乙烯利和茉莉酸处理下GhERF8基因在叶片中上调表达,而在脱落酸处理下GhERF8表达量无显著变化,推测GhERF8可能处于乙烯和茉莉酸信号转导网络中,且表达途径为非ABA依赖途径。
The ethylene-responsive element-binding factor （ERF） gene family is one of the largest transcription factor gene fami- lies in plants. In this study, a novel ERF gene named GhERf8 （GenBank： JN656957） was isolated from our laboratory＇s leaf cD- NA library for Gossypium hirsutum L. The gene encodes a putative of 265 amino-acid protein with a conserved AP2 domain. To understand the function of GhERFS, real-time quantitative PCR （RT-PCR） was used to evaluate the relative expression of GhERF8 at different developmental stages of leaves and tissues. The results showed that GhERf8 could express in all tissues, but expression levels differed. The expression of GhERF8 was the highest in the later stage of leaf maturation. During leaf senes- cence, GhERF8 expression and chlorophyll content were inversely correlated, indicating that GhERP8 could be related to leaf senescence. Under ethephon and jasmonic acid （JA） treatment, the expression of GhERF8 was up-regulated in leaves, but there was no significant change in expression of GhERF8 under abscisic acid （ABA） treatment. These results indicated that GhERF8 could participate in the signal transduction of ethylene and JA, but not in the ABA-dependent pathway.
出处
《棉花学报》
CSCD
北大核心
2012年第6期488-495,共8页
Cotton Science
基金
国家重点基础研究发展计划(2010CB126006)
国家棉花产业技术体系(CARS-18)
关键词
乙烯响应元件结合因子
陆地棉
激素处理
荧光定量PCR
ethylene response factor （ERF）
cotton （Gossypium hirsutum L.）
hormone treatment
real-time fluorescence quanti- tative PCR
