摘要
目的研究节旋藻藻胆蛋白对果蝇体内总抗氧化能力(T-AOC)和丙二醛(MDA)含量的影响。方法采用鄂尔多斯钝顶节旋藻藻胆蛋白(P1)和Chad湖钝顶节旋藻藻胆蛋白(P2)分别配制0.25%、0.50%和1.00%浓度的含蛋白培养基,以普通培养基为对照组、喂饲果蝇并测定各剂量组果蝇体内T-AOC和MDA含量。结果 P1、P2不同剂量组的雄果蝇T-AOC较对照组均显著提高(P<0.05),雌果蝇T-AOC虽提高,但为达到显著性水平。与对照组相比,P1组雌雄果蝇MDA含量明显降低(P<0.05),P2组雄果蝇MDA含量明显降低(P<0.05)。结论节旋藻藻胆蛋白具有增强雄果蝇T-AOC和降低果蝇体内MDA含量的作用。不同产地钝顶节旋藻的藻胆蛋白对果蝇T-AOC和MDA含量的影响不存在差异。
Objective To explore effect of Phycobiliprotein on the total antioxidant capacity （T-AOC） and malondialdehyde （MDA） in vivo of Drosophila melanogaster. Methods The two test groups were respectively fed with culture media contained different dose （0. 25% , 0. 50% and 1.00% ） of P1 which was phycobiliprotein of Arthrospira platensis from alkaline lakes in the Ordos Plateau, and P2 which was phycobiliprotein of Arthrospira platensis from Chad lake. Culture media of control group was general culture media. T-AOC and MDA were detected in Drosophila melanogaster. Results T-AOC activity in male Drosophila melanogaster of P1, P2 groups was higher than that of control group （ P 〈 0. 05 ） and T-AOC in female Drosophila melanogaster was improved in P1, P2 groups, but there was no significant difference between P1, P2 groups and control group. MDA in male and female Drosophila melanogaster in P1, P2 groups were all decreased compared with the control group （P 〈 0.05 ）. Conclusions Phycobiliprotein has the effects of enhancing activity of T-AOC in male Dro- sophila melanogaster and lowering content of MDA in vivo Drosophila melanogaster. There is no difference of effect of phycobiliprotein from different production region on T-AOC and MDA in vivo of Drosophila melanogaster.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第21期4685-4687,共3页
Chinese Journal of Gerontology
基金
内蒙古自然科学基金(2010BS0504)
内蒙古自治区高等学校科学研究项目(NJzy 08025)
关键词
节旋藻
藻胆蛋白
总抗氧化能力
丙二醛
Arthrospira
Phycobiliprotein
Total antioxidant capacity
Malondialdehyde
