摘要
目的探讨艾灸预处理防治阿尔茨海默病(AD)的作用机制。方法健康雄性SD大鼠40只,随机分为正常组、假手术组、模型组和预艾灸组。双侧海马一次性注射聚集态Aβ25～35制作AD模型,艾灸"百会"、"肾俞"、"足三里"进行防治,免疫组化SP法观察大鼠海马CA1区脑源性神经营养因子(BDNF)及其受体TrkB的表达及艾灸干预的影响。结果与正常组及假手术组比较,模型组大鼠海马CA1区BDNF及TrkB的表达明显下降,表现为阳性神经元平均灰度值明显上升(P<0.01);与模型组比较,预艾灸组大鼠海马CA1区BDNF及TrkB的表达明显增加,表现为阳性神经元平均灰度值明显下降(P<0.01)。结论预艾灸治疗可显著增加海马CA1区BDNF及TrkB的表达,从而改善脑内神经元的损伤,起到保护脑细胞的作用。
Objective To investigate the mechanism of Moxibustion pretreatment in prevention and cure of Alzheimer＇s disease （AD）. Methods Rats were randomly divided into normal , sham, model and moxibustion pretreatment groups （ n = 10）. AD rats were induced by injecting β-amyloid25-35 （Aβ325-35） into the double hippocampus. Baihui, Shenshu and Zusanli acupoints were used to prevent and cure AD by moxibustion. Brain-derived neurotrophic factor （BDNF） and its receptor TrkB in hippocampus CA1 zone of rats were detected by immunohistochemistry. Results Compared with normal and sham group, the expression of BDNF and its receptor TrkB in model group were significantly decreased （P 〈 0. 01 ）. Compared with the model group, the expressions of BDNF and TrkB in moxibustion pretreatment group were significantly increased （P 〈 0. 01 ）. Conclusions Moxibustion pretreatment could effectively increase the expressions of BDNF and TrkB in hippocampus CA1 zone, ameliorate the neuron injury of the brain and protect the brain cells.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第21期4663-4665,共3页
Chinese Journal of Gerontology
基金
湖北省教育厅科学技术研究项目(Q20101803)
武汉市科技局青年科技晨光计划项目(201150431126)
湖北中医药大学校级科研项目(中医院字〔2008〕192号)
关键词
阿尔茨海默病
艾灸预处理
BDNF
TRKB
Alzheimer＇s disease
Moxibustion pretreatment
Brain-derived neurotrophic（BDNF）
TrkB
