摘要
目的探讨氧化高密度脂蛋白(oxHDL)及其亚型对人脐静脉内皮细胞(HUVECs)组织因子途径抑制物-1(TFPI-1)表达的影响。方法以天然HDL及其亚型作为对照,HUVECs分别经不同浓度(0,10,20,40,80 mg/L)oxHDL、oxHDL2、oxHDL3孵育24 h,逆转录聚合酶链反应(RT-PCR)、Western印迹分别检测TFPI-1 mRNA及蛋白质表达。结果与对照组比较,oxHDL、oxHDL2、oxHDL3能抑制TFPI-1 mRNA和蛋白表达,其中在oxHDL和oxHDL3各系列浓度中以40 mg/L作用最明显,TFPI-1 mRNA和蛋白表达分别下降了28%、35%和49%、47%;在oxHDL2各系列浓度中以80 mg/L作用最明显,TFPI-1 mRNA和蛋白表达均下降了27%,差异均具有统计学意义(P<0.05);且以oxHDL3的作用最强。结论 oxHDL及其亚型能抑制HUVECs TFPI-1 mRNA和蛋白表达,oxHDL3的作用最强。
Objective To investigate the expression of tissue factor pathway inhibitor-1 （TFPI-1） induced by oxidized high densi- five lipopmtein （HDL） and its subfractions in human umbilical vein endothelial cells （HUVECs）. Methods HUVECs were incubated with oxHDL, oxHDL2, oxHDL3 or their native forms at different concentrations （0,10,20,40,80 mg/L）for 24 h. TFPI-1 mRNA and＂ protein ex- pressions were determined by RT-PCR and Western blot, respectively. Results Compared with control group（ie. HDL, HDL2 or HDL3）, oxHDL, oxHDL2, oxHDL3 significantly decreased the expressions of TFPI-1 mRNA and protein, respectively. TFPI-1 mRNA and protein ex- pressions were lowered by oxHDL by 28% and 35% , respectively at the level of 40 rag/L, by oxHDL2 by 27% and 27% at the level of 80 rag/L, and by oxHDL3 by 49% and 47%, respectively, at the level of 40 mg/L （ all P 〈 0. 05 ）. The effect of oxHDL3 appeared the strongest. Conclusions Ox-HDL and its subfractions could inhibit the expression levels of TFPI-1 mRNA and nrotein in HUVECs.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第21期4660-4662,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助(30971169)
湖南省大学生研究性学习和创新性实验课题(湘教通〔2008〕91号)
湖南省高校科技创新团队资助计划(2008-244)
南华大学留学归国人员科研启动基金资助
关键词
高密度脂蛋白
氧化修饰
组织因子途径抑制物
1
动脉粥样硬化
High-density lipoprotein
Subfractions
Oxidative modification
tissue factor pathway inhibitor（TFPI）-1
Atherosclerosis
