摘要
为研究冠心病患者低密度脂蛋白亚组分颗粒直径的异常特征 ,超速离心分离 37例冠心病患者及 2 6例对照组的血浆低密度脂蛋白 ,采用 2 %～ 16 %聚丙烯酰胺凝胶梯度电泳法分析低密度脂蛋白颗粒直径及电泳图形。发现冠心病患者低密度脂蛋白颗粒直径 ( 2 5 .71± 0 .6 6nm)较对照组 ( 2 6 .41± 0 .95nm)显著变小 (P <0 .0 1) ,两组间A型、B型低密度脂蛋白分布不同 (P <0 .0 5 )。低密度脂蛋白颗粒直径分别同血浆甘油三酯 (r=- 0 .2 9,P <0 .0 5 )和载脂蛋白B(r=- 0 .2 5 ,P <0 .0 5 )呈负相关 ,同高密度脂蛋白胆固醇水平 (r=0 .2 8,P <0 .0 5 )呈正相关。B型低密度脂蛋白中游离胆固醇 蛋白质比值 ( 1.0 2± 0 .45 )较A型 ( 1.5 9± 1.2 1)显著下降 (P <0 .0 5 ) ;低密度脂蛋白颗粒直径同游离胆固醇 蛋白质比值呈正相关 (r=0 .31,P <0 .0 5 )。提示冠心病患者血浆低密度脂蛋白以小颗粒的B型为主 ;
Aim To study size of low density lipoprotein (LDL) particle in patients with coronary artery disease (CHD).Methods Plasma LDL in 37 CHD patients and 26 control subjects were analyzed by 2%～16% non-denaturing polyacrylamide gradient gels. Results The CHD patients had significantly less particle diameters than control subjects(25.71±0.66 nm vs. 26.41±0.95 nm, respectively; P<0.01). The distribution of LDL subfraction pattern was significantly difference between two groups (P<0.05). LDL particle diameter was found negatively correlated with plasma triglycerides (r=-0.29, P<0.05) and apoB (r=-0.25, P<0.05) level, while positively correlated with plasma HDLC level(r=0.28, P<0.05). Free cholesterol to protein ratio of LDL in pattern B was found less than that in pattern A (1.02±0.45 vs. 1.59±1.21, P<0.05), meanwhile a significantly positive correlation was also found between LDL particle diameter and this ratio (r=0.31, P<0.05).Conclusion Small, density pattern B LDL subfraction may be a risk factor for atherosclerosis. Plasma triglyceride may be a factor in regulation of LDL subfraction particle diameter.
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第2期124-126,共3页
Chinese Journal of Arteriosclerosis
基金
江苏省应用基础研究课题!(BJ97111)
