摘要
目的探讨钙网蛋白(calreticulin,CRT)在胆固醇结石患者胆囊组织和胆汁中表达及临床意义。方法利用生物质谱技术鉴定胆固醇结石患者与正常对照组胆囊胆汁的差异表达的蛋白质,通过免疫组织化学和Western blotting验证CRT在胆固醇结石患者胆囊组织和胆汁中表达结果。结果比较蛋白质组学结果显示胆固醇结石患者组CRT表达上调,免疫组织化学和免疫印迹法进一步证实了CRT在胆囊组织及胆汁中高表达。结论 CRT在胆固醇结石患者组的高表达,可能与胆固醇结石的发生、发展及预后有关。
Objective To investigate the expression of calreticulin（CRT） in gallbladder tissue and bile from patients with cholesterol cholelithiasis,and to explore its clinical implication.Methods The differentially expressed proteins of gallbladder bile from cholesterol gallstone patients and control ones were identified by biological mass spectrometry technology.The expression of CRT in gallbladder tissue and bile from patients with cholesterol cholelithiasis was detected with immunohistochemistry and Western blotting.Results The result of comparative proteomics showed that CRT was up-regulated in cholesterol gallstone patients.CRT expressed highly in gallbladder tissue and bile from patients with cholesterol cholelithiasis,which was further confirmed with immunohistochemistry and Western blotting.Conclusion Up-regulation of CRT expression in cholesterol cholelithiasis group may be involved in the formation,development and prognosis of cholesterol gallstone.
出处
《肝胆胰外科杂志》
CAS
2012年第5期377-380,共4页
Journal of Hepatopancreatobiliary Surgery
基金
国家自然科学基金资助项目(30901443
30772111)
江苏省医学重点学科(XK200703)开放课题项目(XK03 200904)
关键词
钙网蛋白
胆固醇
胆结石
calreticulin
cholesterol
cholesterol gallstone
