摘要
目的:观察钙网蛋白在三种疼痛模型大鼠的脊髓中表达的变化,探讨其与疼痛的可能关系.方法:分别制作甲醛炎性痛模型、保留性坐骨神经分支损伤(SNI)模型和慢性缩窄性坐骨神经损伤(CCI)模型,观察大鼠疼痛行为学改变,并分别用免疫组化和Western Blot技术检测大鼠脊髓c-Fos和钙网蛋白的表达.结果:与对照组相比,各模型组大鼠脊髓后角c-Fos阳性神经元增多(P<0.05);SNI模型组和CCI模型组大鼠脊髓钙网蛋白的表达均上调(P<0.05),甲醛炎性模型组大鼠脊髓钙网蛋白变化无统计学意义(P>0.05).结论:脊髓钙网蛋白的表达上调与SNI和CCI模型的慢性神经病理痛发生过程有关.
AIM： To detect the change of rat spinal calreticulin expression levels in three pain models and to investigate the possible involvement of calreticulin in pain development. METHODS： Formalin inflammatory pain model, spared never injury（SNI） model, and chronic constriction injury（CI） model were established. Then, the behavioral changes were observed and evaluated and c-Fos and calreticulin expressions in the spinal cord of rats were detected with immunohistochemistry and Western Blot, respectively. RESULTS： Compared with control groups, quantity of c-Fos positive neurons significantly increased in spinal dorsal horn of rats with formalin injection, SNI or CCI （ P 〈 0.05）; calreticulin expression levels were up-regulated significantly in spinal cord of rats with SNI and CCI （ P 〈 0.05 ）, but not in spinal cord of rats with formalin injection （ P 〉 0.05 ）. CONCLUSION： Up-regulation of calreticulin expression in spinal cord is involved in the developmeut of chronic neuropathic pain induced by SNI and CCI.
出处
《第四军医大学学报》
北大核心
2007年第14期1268-1271,共4页
Journal of the Fourth Military Medical University
关键词
疼痛
脊髓
钙网蛋白
pain
spinal cord
calreticulin
