摘要
[目的]对香附黄酮的抗氧化作用进行研究。[方法]以浓度95%乙醇为溶剂,水浴回流提取香附中的黄酮,然后采用比色法测定香附黄酮对DPPH.、.OH和O2-.3种自由基的清除作用以及还原能力。[结果]香附黄酮对3种自由基都有明显的清除作用,清除能力大小顺序为DPPH.>.OH>O2-.;清除率与浓度存在明显的量效关系,当黄酮浓度为0.4 mg/L时,其对O2-.的清除率为25.5%,DPPH.的为67.0%,.OH的为28.5%;还原Fe3+能力随黄酮的浓度增加而增强。[结论]香附黄酮具有较强的抗氧化活性。
[Objective] To study the antioxidant effects of flavonoids in RHIZOMA CYPER.[Method] Taking 95% ethanol as the solvent,flavonoids were extracted from RHIZOMA CYPER by water bath reflux.Then,reduction ability and scavenging effect of RHIZOMA CYPER flavonoids on three free radicals（DPPH·,·OH and O-2·） were researched by colorimetric method.[Result] flavonoids in RHIZOMA CYPER had significant scavenging effects on the three free radicals;and their scavenging abilities were in the order of DPPH· ·OH O-2·.There were significant dose-effect relationship between concentration and scavenging rate.When the flavonoids concentration was 0.4 mg/ L,the scavenging rates to O-2·,DPPH· and ·OH were 25.5%,67.0% and 28.5%,respectively.Fe3＋ reducing ability enhanced as the concentration increased.[Conclusion] Flavonoids in RHIZOMA CYPER had relatively strong antioxidant activity.
出处
《安徽农业科学》
CAS
2012年第33期16117-16119,共3页
Journal of Anhui Agricultural Sciences
基金
右江民族医学院自治区级大学生创新训练计划项目资助
编号:QJCX201309
