摘要
目的 纯化EB病毒特异性DNA酶 (EBV -DNase)为鼻咽癌血清学诊断方法—血清EBV -DNase抗体的检测奠定基础。方法 采用多重纤维素柱层析对用联合化学诱导的方法制备的EBV -DNase粗提物进行纯化。结果 在SDS -PAGE检测中 ,从粗提物到纯化终产物 ,蛋白质带逐渐减少直至单一条带 ,其分子量为 52kD ,其对应的每毫克蛋白质酶单位分别为 5.4 6 ,4 9.19,134.86 ,391.2 5,96 7.78。酶纯化倍数为 177.2。结论 纤维素柱层析方法较满意地将EBV -DNase从粗提物中分离纯化 ,得到理想的纯化产物 ,为鼻咽癌血清EBV
Objective To purify Epstein-Barr virus-specific DNase (EBV-DNase) for further establishment of detection of EBV-DNase antibody in nasopharyngeal carcinoma (NPC) sera. Methods The EBV-DNase extract obtained through combined chemical induction was purified with multistage of gradient linear cellulose column chromatography. Results SDS-PAGE showed that the protein bands were gradually decreased to a single one with a molecular weight of 52kD from the crude extract to the terminal purified product. The immunoradiological determination showed that the units per milligram protein (U/mg) were 5.46, 49.19, 134.86, 391.25 and 967.78 respectively. The enzyme activity was enhanced by 177.2 folds. Conclusion Gradient linear cellulose column chromatography can satisfactorily purify EBV-DNase from the crude cellular extract.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2000年第1期28-31,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
