摘要
采用免疫组织化学ＡＢＣ法对３０耳中耳胆脂瘤、１１耳健康外耳道皮肤进行表皮生长因子受体（ＥＧＦＲ）定位，用酶联免疫电转移印迹技术（ＥＩＴＢ）检测８耳中耳胆脂瘤、４耳健康外耳道皮肤中ＥＧＦＲ。结果：１３０耳中，２９耳上皮、２４耳成纤维细胞、单个核细胞及血管内皮细胞显示ＥＧＦＲ阳性，免疫着色主要定位于细胞膜及细胞浆，且从基底细胞层到角质层逐渐减弱；外耳道健康皮肤仅散在基底细胞和棘细胞及６耳血管内皮细胞呈弱阳性。２８耳胆脂瘤标本中有６耳在１７０ＫＤ处出现阳性蛋白带，所有外耳道健康皮肤未见任何免疫反应阳性产物。
Immunohistochemical localization and immunobiochemical detection of epidermal growth factor receptor (EGFR) in middle ear cholesteatoma (MEC) and normal external auditory canal (EAC) were adopted with ABC and enzyme-linked immunoelectrotransfer blot (EITB) technique The results were as follows: 1 Of the 30 ears of the MEC group positive immunoreactivity localized mainly in cell membrane and cytoplasm was revealed throughout the whole epithelial layers of 29 ears, and fibroblasts and mononuclear cells as well as vascular endothelial cells in 24 ears. Only discrete basal cells and spinous cells of all cases and vascular enthothelial cells of six in eleven ears of EAC group showed faint immunoreactivity 2 A clearly immunoreactive protein band corresponding to the site of 170KD standard protein was found in six of eight ears of MEC, while none of all four ears of EAC showed positive immunoreactive protein band The possible role played by EGFR in MEC was discussed
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
1996年第2期85-88,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
关键词
中耳
胆脂瘤
免疫组织化学
表皮生长因子
受体
Middle ear\ \ Cholesteatoma\ \ Immunohistochemistry Epidermal growth factor receptor Enzyme-linked immunoelectrotransfer blot
