摘要
目的 观察长期被动吸烟所致大鼠肺损伤情况及其对体内一氧化氮、白细胞介素 6、8(IL 6、IL 8)影响作用如何。方法 采用生化分光光度法测定NO-2 /NO-3 含量 ,代表大鼠体内NO水平。用酶联免疫标记法测定IL 6、IL 8含量。结果 单纯吸烟组 ,血清、支气管肺泡灌洗液、肺组织中的NO含量均明显低于正常对照组。IL 6、IL 8含量均高于正常对照组。结论 长期被动吸烟可引成肺组织一定程度的损伤 ,并使肺组织内皮细胞分泌NO减少 ,肺血管受到一定的损伤。
Objective A rat model with chronic bronchitis was replicated by passive inhalation of cigarette smoking fume to study its long term effects on lung injury and nitric oxide(NO), interluekin 6 (IL 6), interleukin 8 (IL 8). Methods Levels of nitrogen dioxide (NO 2) and nitrogen trioxide (NO 3) were measured with spectrophotometry in rats indicating their level of nitric oxide (NO). Levels of IL 6 and IL 8 were determined by enzyme linked immunosorbent assay (ELISA). Results Levels of NO in serum, bronchial alveolar lavage fluid (BALF) and lung tissue in the smoking group were significantly lower than those in the normal controls ( P < 0 01). But, levels of IL 6 and IL 8 were higher in the smoking group than those in the controls. Conclusion Long term passive smoking could cause injury of lung tissue to certain extent, reduction in secretion of NO in endothelial cells and damage to pulmonary vessels.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第2期104-105,共2页
Chinese Journal of Preventive Medicine
