摘要
目的探讨经香烟烟雾溶液染毒的人正常肺间质细胞和人肺腺癌细胞的DNA损伤及其修复效应。方法体外培养人胚肺成纤维细胞(HLF)和人肺腺癌A549细胞,以二甲基亚砜(DMSO)和磷酸缓冲液(PBS)作为吸收液,采集香烟主流烟雾。用四甲基偶氮唑盐(MTT)法测定香烟烟雾-DMSO吸收液和香烟烟雾-PBS吸收液(分别简称DMSO烟液和PBS烟液)的1/2、1/4、1/8和1/16倍稀释液和原液对HLF细胞和A549细胞的毒性(分别以DMSO和PBS为阴性对照),以无明显细胞毒性的浓度进行彗星实验(分别以DMSO和PBS为阴性对照,以重铬酸钾为阳性对照),测定细胞的DNA损伤及修复情况。结果DMSO烟液原液及其1/2稀释液染毒的细胞存活率低于80%,而PBS烟液染毒的细胞存活率均高于80%。DMSO烟液的1/4、1/8、1/16倍稀释液以及PBS烟液的1/2、1/4、1/8和1/16倍稀释液和原液对HLF细胞和A549细胞的DNA损伤与阴性对照组相比,差异均具有统计学意义(P<0.01),且存在明显的剂量-效应关系。DNA损伤在两种细胞间差异无统计学意义(P>0.05),但DMSO烟液所致的DNA损伤高于PBS烟液(P<0.01)。细胞在去除受试物后培养30min时开始修复,随着修复时间的延长,拖尾细胞数减少,DNA迁移长度缩短(P<0.05),且A549细胞修复得更快(P<0.05)。结论DMSO烟液的细胞毒性和遗传毒性均高于PBS烟液。香烟烟雾对HLF细胞和A549细胞的DNA损伤差异不明显,A549细胞的DNA损伤修复能力较HLF细胞强。
Objective To study the DNA damage and repair of normal lung interstitial cells and human lung adenocarcinoma cells exposed to cigarette smoke. Methods Cultured human embryo hmg fibroblasts （HLF） and human lung adenocarcinoma A549 cells. Mainstream smoke was collected by using dimerhyl sulfoxide （DMSO） and phosphate buffer solution （PBS） as absorbents. MTF assay was used to test the cytotoxicity of the solutions of cigarette smoke, then selected the concentrations of the solutions with no obvious eytotoxicity to treat cells and detected DNA damage and repair by comet assay. Results As treated with original solutions or 1/2 dilution of DMSO cigarette smoke solutions only, the Viability of cells was below 80%, but it was beyond 80% when treated with PBS solutions. The results showed that a significant difference of DNA damage was seen between the treated groups and negative control groups （P〈0.01）, and the damage of DNA was dose-dependent. No significant difference was shown in DNA damage between HLF and A549 （P〉0.05）,but the DNA damage caused by DMSO solutions was worse than PBS solutions significantly （P〈0.01）, Cultured cells began to repair at 0.5 h after ending exposure, the comet rate and tail length decreased （P〈 0.05）. A549 cells repaired more rapidly（P〈0.05）. Conclusion The solutions of cigarette smoke absorbed by DMSO has stronger cytoroxicity and genocoxieity than PBS. Between HLF and A549 there is no significant difference in cigarette smoke-induced DNA damage but A549 are more competent to repair the damaged DNA than HLF.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第2期142-145,共4页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30571535)
