摘要
本研究利用体外培养人体肠道菌转化黄芩苷,探索转化方法及模型;用醇沉法提取了黄芩苷转化酶,即β-D-葡萄糖醛酸苷酶,并探讨了酶促影响因素;通过高效液相色谱检测产物黄芩素。经实验确定,黄芩苷转化培养液经超声波处理后,在转化液中有黄芩素检出。实验得知,转化酶为胞内酶,该酶的最适反应温度为55℃,最适pH为6.0,Ca2+、Mg2+和Cu2+对酶促反应具有促进作用,而Fe2+则具有抑制作用,Zn2+浓度在l mmol/L时起促进作用,在5 mmol/L时起抑制作用。
The characteristic of human intestinal bacteria biotransformation system was studied. The enzyme （β-D-glucuronidase） was isolated by Alcohol precipitation,and the factors on enzymatic properties were analyzed. The product was identified as baicalein by high performance liquid chromatography （HPLC）. After ultrasonic treatment on cultivation medium, the baicalein could be extracted. At pH 6.0 and 55 ℃, the activity of β-D-glucuronidase reached maximum, and was activated by Ca^2＋ ,Mg^2＋ and Cu^2＋ ,but inhibited by Fe^2＋ ;at 1 mmol/L Zn^2＋ played a catalytic role,but an inhibitory factor at 5 mmol/L.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第10期1437-1440,1392,共5页
Natural Product Research and Development
基金
陕西省教育厅专项科研项目(09JK453)
陕西省科技厅工业攻关项目(2008k07-14)
