摘要
还原型维生素C在 2 43 8nm下有最大吸收峰 ,在 0～ 45 0 μg/mL范围内具有良好的线性。本研究以Cu2 + 作催化剂 ,利用溶解氧 ,将在 2 43 8nm处有最大吸收的还原型维生素C选择性的氧化为 2 43 8nm处无吸收峰的氧化型维生素C ,实现了对样品中各种紫外干扰成分的本底校正 ,建立了一种测定果蔬中还原型维生素C的新方法。运用该方法实际测定了草莓、西红柿等样品中的还原型维生素C的含量 ,回收率在 97 6 %～ 1 0 0 8%之间 ,测定结果与荧光法基本一致。
The ascorbic acid has maximum absorption at 243 8 nm A liner relationship was observed in the range of 0～450 μg/mL and the calibrated equation was y=0 112 6 x +0 002 4 with correlation coefficient of 1 000 0 In this paper,using Cu 2+ as catalyst,the ascorbic acid could be oxidixed into oxidization type Vc which had no absorption at 243 8 nm selectively by solvent oxygen,then the absorption in ultraviolet band of disturbing material could be corrected,after that,a new method was established to determined the reduction type Vc in fruits and vegetables Using this method,some kinds of samples such as tomato and strawberry were determined and the coefficient of recovery was in the range of 97 6%～100 8%,the results was the same as the method of fluorescence spectrometer
出处
《河北职业技术师范学院学报》
2000年第1期41-44,共4页
Journal of Hebei Vocation-Technical Teachers College
关键词
维生素C
测定方法
紫外分光光度法
水果
蔬菜
ultraviolet spectrophotometry
ascorbic acid
fruit and vegetable
determining methods
