摘要
为获得L-精氨酸高产菌株,以实验室初步鉴定为格兰氏阳性细菌,菌落特征为金黄色的产全氨基酸菌株为出发菌株,利用紫外线(UV)诱变和精氨酸结构类似物羧甲基半胱氨酸(羧甲司坦)进行复合筛选,得到1株L-精氨酸高产诱变菌株。对所筛选到的高产诱变菌株进行160r/min,30℃摇瓶发酵90h,测得该菌株L-精氨酸的产量为78.79μg/3ml,与出发菌株相比,L-精氨酸的产量提高约3.71倍。经多批次发酵试验进一步验证,所筛选的高产L-精氨酸菌株具有较好的遗传稳定性,此研究不仅对筛选L-精氨酸高产菌株具有一定参考和借鉴意义,对筛选其他类型氨基酸高产菌株也可提供一定参考。
In order to obtain a higher production strain of L-arginine,a Gram-positive strain identified by our lab was used as an initial strain in this study.The initial strain has a flora colony characteristic,and it can produce the whole amino acids.Ultraviolet irradiation and one structural analog called Carboxymethyl-L-Cysteine of arginine were used to select higher production strain,finally a promising higher production strain was identified,named as mutant-3.The fermentation experiment results showed that under the condition of 160r/min and 30℃ for 90h,the amount of L-arginine was 78.79μg/3ml,which was about 3.71 times higher than that of initial strain.Mutant-3 was approved to be a promising strain with better production stability by a series of fermentation experiments.This study could provide some references for screening arginine producer strain,and give some important uses for selecting other amino acid producer strain.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第6期879-883,共5页
Journal of Nuclear Agricultural Sciences
基金
河南省科技攻关项目(112102310443)
关键词
L-精氨酸
高产菌株
紫外线诱变
复合筛选
L-arginine
higher production strain
UV irradiation
complexed screening
