摘要
以谷氨酸棒杆菌(Corynebacterium glutam icum)ATCC138761为诱变出发菌株,经紫外线(UV)和亚硝基胍(NTG)逐级诱变处理和选育,获得一株能够积累L-精氨酸的菌株UN100-12(SGr,AEr)。在以葡萄糖为碳源、以硫酸铵为氮源的培养基中直接发酵4d,产酸可达16.6 g/L,并具有较好的遗传稳定性。
A L-arginine-producing mutant UN100-12(SG^(r),AE^(r)) was derived from Corynebacterium glutamicum ATCC138761 by combination treatment with ultraviolet(UV) and N-methyl-N-nitro-N-nitrosoguanidine(NTG). It could accumulate 16.6g/L L-arginine in the medium containing glucose as carbon source and ammonium sulfate as nitrogen source, when it was culrured at 30℃ for 4 days. And also this mutant has good stability of descendiblity of L-arginine.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第3期46-50,共5页
Microbiology China
关键词
L-精氨酸
谷氨酸棒杆菌
发酵
诱变育种
L-arginine, Corynebacterium glutamicum, Fermentation, Breeding
