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对亚胺培南不敏感的肺炎克雷伯菌耐碳青霉烯类药物的机制研究 被引量：5

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摘要 目的研究31株对亚胺培南不敏感的肺炎克雷伯菌的耐药基因及外膜蛋白的改变。方法纸片扩散法筛选对亚胺培南不敏感的肺炎克雷伯菌,进行金属酶表型初筛试验、常见耐药基因PCR及测序、外膜蛋白分析,研究其耐药基因及外膜蛋白改变。结果 31株肺炎克雷伯菌中,22株产KPC-2酶,1株产IMP-4酶,产碳青霉烯酶菌株外膜蛋白均有改变。结论 KPC型碳青霉烯酶仍为肺炎克雷伯菌对碳青霉烯类抗生素不敏感的主要原因,但金属酶IMP的出现,提示应加强对金属酶的检测。
作者 宁明哲 沈翰 张之烽 周万青 张葵
机构地区 南京大学医学院附属南京市鼓楼医院检验科
出处 《临床检验杂志》 CAS CSCD 北大核心 2012年第9期706-708,共3页 Chinese Journal of Clinical Laboratory Science
基金 南京市卫生局资助项目(YKK09115)
关键词 碳青酶烯酶 肺炎克雷伯菌 耐药
分类号 R446.5 [医药卫生—诊断学] R37 [医药卫生—病原生物学]
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参考文献14

  • 1Yigit H, Queenan AM, Anderson GJ, et al. Novel carbapenem-hy- drolyzing β-1actamase, KPC-1, from a carbapenem-resistant strain of KlebsieUa pneumonia [ J ]. Antimicrob Agents Chemother, 2001,45 (4) :1151-1161.
  • 2Naas TP, Nordmann P, Vedel G,et al. Plasmid-mediated carbapen- em-hydrolyzing β-1actamase KPC in a Klebsiella pneumonia isolated from FRANCE[J]. Antimicrob Agenets Chemother,2008,49(10) : 4423 -4424.
  • 3Navon-Venezia S, Chmelnitsky I, Leavitt A, et al. Plasmid-meda-ted imipenem-hydrolyzing enzyme KPC-2 among multiple carbapen- em-resistant Escherichia coli clones in Israel[ J]. Antimicrob Agenets Chemother,2006,50(9) : 3098-3101.
  • 4Villegas MV, Lolans K, Correa A, et al. First detection of the plasmid-mediated class A carbapenemase KPC-2 in clinical isolates of Klebsiella pneumonia from South Amercia [ J ]. Antimicrob Agents Chemother, 2006,50 ( 8 ) : 2880 -2882.
  • 5Cuzon G, Nass T, Demachy MC, et al. Plasmid-mediated imipenem- hydrolyzing 13-1actamase, KPC-2 in Klebsiella pneumonia isolate from Greece [ J ]. Antimicrob Agenets Chemother, 2008,52 ( 8 ) : 796 -797.
  • 6M100-S21. Performance Standards for Antimicrobial Susceptibility Testing; Twenty First Informational Supplement[ S]. CLSI, 2011.
  • 7Smith Moland E, Hanson ND, Herrera VL, et al. Plasmid-mediated carbapenem-hydrolysing β-1actamase, KPC-2, in Klebsiella pneumonia isolates [ J]. Antimicrob Agents Chemother, 2003, 51 (3) :711-714.
  • 8Senda K, Arakawa Y, Ichiyama S, et al. PCR detection of metallo- β-lactamase gene (blaiMP ) in gram-negative rods resistant to broadspectrum β-1actamases[ J]. J Clin Microbiol, 1996, 34(12) : 2909- 2913.
  • 9Tsakris A, Pournaras S, Woodford N, et al. Outbreak of infections caused by Pseudimonas aeruginosa producing VIM-1 carbapenemase in Greece [ J]. J Clin Microbiol, 2000,38 ( 3 ) : 1290-1292.
  • 10Queen AM, Torres-Viera C, Gold HS, et al. SME-type carbapenem-hydrolyzing class A β-1actamasefrom grographically diverse Serratia marcescens strains [ J ]. Autimicrob Agents Chemother, 2000, 44 ( 11 ) : 196-199.

二级参考文献33

共引文献52

同被引文献42

  • 1Neuberger A, Oren I, Sprecher H. Clinical impact of a PCR assay for rapid identification of Klebsiella pneumoniae in blood cultures[ J ]. J Clin Mic'robiol,2008,46( 1 ) :377-379.
  • 2Chuang YP, Fang CT, I,ai SY, et al. Genetic determinants of'eapsu- lar serotype KI of Klebsiella pneumoniae causing primal' pyogenic liver abscess[ J ]. J Infect Dis,2006, 193 ( 5 ) :6454~54.
  • 3Turton ,IF, Baklan H, Siu LK, et al. Evaluation of a multiplex PCR for detection of serotypes KI, K2 and K5 in Klebsielltz sp. and com- parison of isolates within these serotypes[ J ]. FEMS Micrnbiul Lett, 2008,284( 2 ) :247-252.
  • 4Turton JF, Per C, Elgohari S, et al. PCR characterization and typ- ing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and vimlenee gene targets[ J]. J Med Miero- bio1,2010,59 ( Pt 5 ) :541-547.
  • 5Brisse S, Fevre C, Passet V, et al. Virulent clones of Klebsiella pneumoniae : identification and evolutionary scenario based on gencm-ic and phenotypic characterization [ J ]. PLoS One, 2009, 4 ( 3 ) : e4982.
  • 6Turton JF, Perry C, Elgohari S, et al. PCR characterization and typ- ing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets [ J ]. J Med Micro- bio1,2010,59 ( Pt 5 ) :541-547.
  • 7Hsieh PF, Lin TL, Lee CZ, et al. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess[ J]. J Infect Dis,2008, 197 (12) : 1717-1727.
  • 8Pournaras S, Poulou A, Voulgari E, et al. Detection of the new met- allo-beta-lactamase VIM-19 along with KPC-2, CMY-2 and CTX-M-15 in Klebsiellapneumoniae[J].J Antimicrob Chemother,2010,65 ( 8 ) : 1604-1607.
  • 9Qi Y, Wei Z, Ji S, et al. ST11, the dominant clone of KPC-produ- eing Klebsiella pneumoniae in China[ J]. J Antimicrob Chemother, 2011,66(2) :307-312.
  • 10Naas T,Nordmann P,Vedel G,et al.Plasmid-media-ted carbapenem-hydrolyzing beta-lactamase KPC in a Klebsiella pneumoniae isolate from France[J].Anti-microb Agents Chemother,2005,49(10):4423-4424.

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临床检验杂志
2012年 第9期

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