摘要
目的研究半乳凝素3（galectin-3）在胰腺癌细胞株中的表达及对人胰腺癌细胞株SW1990增殖和侵袭能力的影响。方法采用免疫细胞化学法和RT-PCR方法检测胰腺癌细胞株SW1990、PANC1、AsPC-1的galectin-3蛋白和mRNA表达。分别应用1、2、3、5μg／mlgalectin-3单抗处理SW1990细胞24、48、72h，采用CCK-8试剂盒检测细胞的增殖，Transwell小室检测细胞的侵袭能力。结果胰腺癌SW1990、PANC1、AsPC-1细胞均有ga｜ectin-3mRNA和蛋白表达。galectin-3单抗呈浓度和时间依赖性抑制SW1990细胞的增殖及穿膜细胞数。培养72h时2、3、5μg／mlgalectin-3单抗的细胞生长抑制率分别为19．8％、29．9％和42．7％，与对照组比较差异均有统计学意义（P值均〈0．05）。3μg／mlgalectin-3单抗组的穿膜细胞抑制率为37．1％，与对照组的10．4％差异有统计学意义（P〈0．05）。结论galectin-3在胰腺癌细胞中高表达，用抗体中和galectin-3能抑制SW1990细胞的增殖和侵袭能力。
Objective To investigate the expression of galectin-3 in pancreatic cancer cell and its effect on the proliferation and invasion of SW1990. Methods Immunocytochemistry and semi-quantitative RT- PCR was used to detect the expression of galectin-3 protein and mRNA in SW1990, PANC1 and ASPC-1 cell lines. Galectin-3 mono-antibody of different concentrations （ 1, 2, 3, 5 μg/ml） was used to treat SW1990 cells for 24, 48, 72 h, CCK-8 kits were used to detect the proliferation in SW1990 cells; Transwell chamber was used to study the invasion in SW1990. Results Expression of galectin-3 protein and mRNA was present in SW1990, PANC1, and ASPC-1. Galectin 3 mono-antibody inhibited the proliferation and number of invasive cells in a dose and time dependant manner. The inhibitory rates at 72 h were 1 9.8%, 29.9% and 42.7% in 2, 3, 5 μg/ml galectin 3 mono-antibody groups, the difference among them and control group was statistically significant （P 〈 0.05）. The inhibite rate of permeating membrane cells in 3 μg/ml galectin-3 mono antibody was 37.1% , the difference between this group and control group was statistically significant （ P 〈 0.05 ）. Conclusions Galectin-3 is highly expressed in pancreatic cancer cells. Galectin-3 antibody can inhibit the proliferation and migration capability of SW1990 cells.
出处
《中华胰腺病杂志》
CAS
2012年第5期320-322,共3页
Chinese Journal of Pancreatology
基金
江苏省创新团队医学领军人才项目（LJ201135）
关键词
胰腺肿瘤
半乳糖凝集素3
细胞增殖
肿瘤浸润
Pancreatic neoplasms
Galectin-3
Cell proliferation
Neoplasm invasiveness
