摘要
目的:探讨脂联素基因单核苷酸多态性+45T/G及+276G/T与妊娠期糖尿病(GDM)及妊娠结局的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对158例GDM孕妇(GDM组)、128例妊娠期糖耐量异常孕妇(GIGT组)及120例糖耐量正常孕妇(对照组)脂联素基因启动子多态性位点(+45T/G和+276G/T)进行分析,并随访分析脂联素基因单核苷酸多态性与相应妊娠结局的关系。结果:①单核苷酸多态性+45T/G:3组基因型频率比较,差异均无统计学意义(P>0.05);GDM组和GIGT组的G等位基因频率均高于对照组(P<0.05);携带G等位基因(杂合子TG型+纯合子突变GG型)的GDM组和GIGT组孕妇,其新生儿低血糖、巨大儿及新生儿窒息的发生率均明显高于未携带G等位基因(野生TT型)者(P<0.05);②单核苷酸多态性+276G/T:3组间的基因型及等位基因频率分布比较,差异均无统计学意义(P>0.05);3组间携带突变T基因(杂合子GT型+纯合子突变TT型)的巨大儿、新生儿低血糖及新生儿窒息发生率与无突变T基因(野生GG型)者相比,差异均无统计学意义(P>0.05)。结论:脂联素基因单核苷酸多态性+45T/G与GDM的发生存在一定关联,亦与其妊娠结局有关;单核苷酸多态性+276G/T与GDM的发生及妊娠结局无明显关联。
ObJecUve： To study the association of adiponectin gene single nucleotide polymorphism （SNP） ＋ 45 T/G and SNP ＋ 276G/T with gestational diabetes mellitus and pregnant outcomes. Methods： SNP ＋45 T/G and SNP ＋276G/T were analyzed by PCR-RFLP technology on 158 pregnant women with gestational diabetes mellitus （GDM） and 128 pregnant women with gestational impaired glucose tolerance （GIGT） and 120 pregnant women with normal glucose tolerance （NGT） as control group. Meanwhile,preg- nancy outcomes were followed up. Results：~For SNP ＋45T/G binding sit, there was no significant differ- ence in the distribution of genotype in the GDM group,the GIGT group and the control group （all P 〉0.05）, the frequencies of G allele in the GDM group and GIGT group were higher than that in the control group （ P 〈 0.05）. The incidences of fetal macrosomia, neonatal hypoglycemia and neonatal asphyxia in the GDM group and GIGT group with G allele were all significantly higher than those without G allele （P 〈0.05）. ~For SNP ＋276 G/T binding sit,there was no significant difference in the distribution of genotype and frequencies of allele in the GDM group, the GIGT group and the control group （ P 〉 0.05）. There were no significant differences in the incidences of macrosomia, neonatal hypoglycemia and neonatal asphyxia in different groups with or without mutant T gene （ P 〉 0.05）. Conclusions： SNP ＋ 45T/G , but not for SNP ＋ 276G/T, has some relationship with the occurrence of GDM and its pregnancy outcome.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2012年第9期743-747,共5页
Journal of Practical Obstetrics and Gynecology
关键词
妊娠期糖尿病
脂联素
基因多态性
妊娠结局
Gestational diabetes mellitus
AdiponecUn gene
Single nucleotide polymorphism
Pregnancy outcome
