摘要
建立杜仲叶多成分的HPLC含量测定方法。建立的色谱系统为:用C18色谱柱,以含0.1%甲酸的乙腈和水为流动相,柱温30℃,波长270 nm,流速1 mL/min。通过对杜仲叶中7种成分(绿原酸、槲皮素-3-O-α-L-吡喃阿拉伯糖基-(1→2)-β-D-葡萄糖基苷、芦丁、异槲皮苷、紫云英苷、槲皮素、山奈酚)的含量测定,确定药材质量的优劣。本方法可使7种成分有效分离,在各自的检测范围内线性良好,精密度和重复性良好,加样回收率范围在99.03%到107.1%之间。经对10批次杜仲叶的测定,认为该方法可以准确地反映杜仲叶质量的优劣。
A high-performance liquid chromatographic method was established to evaluate the quality of Eucommiae Folium with a simultaneous determination of seven compounds,chlorogenic acid,quercetin 3-O-α-L-arabinopyranosyl-（1→2）-β-D-glucopyranoside,rutin,isoquercitrin,astragaline,quercetin and kaempferol.The samples were separated on a Hedera-C18 column with gradient elution of acetonitrile and 0.1% methanoic acid at a flow rate of 1.0 mL/min and detected at 270 nm.The seven compounds were completely separated within 45 min and the determinations indicated good linearity.The average recoveries varied from 99.03% to 107.05%.This method was validated with respect to precision,repeatability and accuracy,and was successfully applied to the quantification of seven compounds in 10 commercial samples of Eucommia ulmoides Oliv..
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2012年第5期435-437,共3页
Journal of China Pharmaceutical University
关键词
杜仲叶
绿原酸
黄酮
黄酮苷
HPLC
Eucommiae Folium
chlorogenic acid
flavonoids
flavone glycosides
HPLC
