摘要
为探讨中国野生刺葡萄冬剪枝条的多酚类物质含量及其抗氧化活性,以7种中国野生刺葡萄冬剪枝条为研究对象,利用超声波辅助传统有机溶剂提取法提取多酚物质。分别测定粗提液中总酚、总黄酮、原花色素的含量,采用体外抗氧化活性法测定其DPPH自由基清除能力、羟自由基清除率、铜离子还原力和总还原力,并分析其相关性。结果表明:枝条中总酚、总黄酮、总原花色素含量(以干基计)分别为24.68～32.76、51.03～72.63、36.69～44.41mg/g。DPPH自由基清除率、羟自由基清除率、铜离子还原力和总还原力分别为23.62～28.02mg/g、31.51%～36.11%、47.19～73.28mg/g和39.76～53.80mg/g。中国野生刺葡萄枝条具有较高的酚类物质含量和抗氧化性能,其中还原力与总酚、总黄酮、原花色素含量有较高的相关性(R2>0.9),是具有良好开发前景的天然抗氧化剂及生物药品基料。
In order to explore their polyphenolic content and antioxidant activity, polyphenolics were extracted from pruned branches of seven varieties of wild spine grape trees in winter collected from a resource garden in Jiangxi province by ultrasonic-assisted organic solvent extraction technique. The contents of total phenols, total flavonoids and proanthocyanidins in crude extracts obtained from these materials were determined. Meanwhile, the crude extracts were analyzed for in vitro DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, cupric reducing power and total reducing power and correlation analysis of these antioxidant properties was carried out. The results revealed that the contents of total phenols, total flavonoids and proanthocyanidins in wild spine grape branches were in the range of 24.68-32.76, 51.03-72.63, and 36.69-44.41 mg per gram of dried sample, respectively. Dried wild spine grape branches from seven varieties ranged from 23.62 to 28.02 mg/g in DPPH radical-scavenging capacity, from 31.51% to 36.11% in hydroxyl radical-scavenging capacity, from 47.19 to 73.28 mg/g in cupric reducing power, and from 39.76 to 53.80 mg/g in total reducing power. Thus, wild spine grape branches are rich in phenolics and have a strong antioxidant effect. Furthermore, a high correlation of reducing power with the contents of total phenols, total flavonoids and proanthocyanidins was observed （R^2 〉 0.9）. In conclusion, wild spine grape branches can provide a promising base material for natural antioxidants and biopharmaceuticals.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期159-163,共5页
Food Science
关键词
刺葡萄
葡萄枝条
多酚类物质
总黄酮
原花色素
抗氧化活性
18tis davidiiFoex., grapevine cane. polyphenol
flavonols
proanthocyanidin
antioxidant activity
