摘要
从金针菇Flammulina velutipes(Curt.ex Fr.)Sing.子实体中提取水溶性多糖,经乙醇分级,DEAE-Sephadex A-25纯化,得PA_5DE。以聚丙烯酰胺凝胶电泳和凝胶柱Sepharose 4B层析证明是化学均一性多糖,分子量是47.1万。用GLC、I.R、~13C-N.M.R.分析表明含有D-葡萄糖、D-甘露糖、D-岩藻糖。PA_5DE分子可能具有分支结构,含β-型糖苷键,存在β(1→3)和β(1→6)型糖苷键连接,并有抑制肿瘤S-180的活性。
The polysaccharide PA5DE was isolated from the fruit bodies of flammulina velutipes (Curt, ex Fr.)Sing. and further purifed by ethanol fractionation and DEAE-Sephadex A-25 chromatography.The PA5DE was shown chemical homogenerous by polyacrylamide gel electrophor-esis and gel-filtration (Sepharose 4B) . The average molecular weight of PA5DE was 4.71×105.The structure of PA5DE was investigated by G.L.C., I.R., and C13 -N. M.R. spectroscopy, The results indicated that the PA5DE was composed of D-glucopyranose, D-mannopyranose and L-fucopyranose, in the molar ratio of 15.83 :1.70: 1,00, and might possess β(l→3) and β(1→6) glucopyransyl linkage.The polysaccharide PA5 showed some inhibitory activity against implanted Sarcoma 180 solid tumor in mouse.
关键词
食用菌
金针菇
子实体
金针菇多糖
提取
性质
Fruit bodies of Flammulina velutipes (Curt, ex Fr.) Sing, Polysac-charide of FLammulina velutipes (Curt, ex Fr.) Sing,
