摘要
检测干细胞因子HIWI在食管鳞癌组织中的表达情况，分析其表达与临床病理及预后的关系，为食管癌的早期诊断和预后提供理论依据。方法：采用免疫组织化学法检测HIWI在153例食管鳞癌组织中的表达情况，并结合食管鳞癌患者10年随访资料，分析其表达与临床病理及预后的关系。结果：免疫组织化学结果显示，HIWI食管鳞癌细胞胞质和胞核中均有表达，其在食管鳞癌细胞胞浆的表达水平与肿瘤的浸润深度（P=O．035，X2=8．589）和组织学分级（P=O．011，X2=9．075）呈正相关。Ka-plan-Meier生存分析显示，肿瘤细胞胞质高表达HIWI的患者的总生存情况明显差于胞浆低表达HIWI的患者（P〈0．001，X2=17．05）。结论：干细胞因子HIWI在食管鳞癌细胞中有不同程度的表达，且其在胞质中的表达水平是影响食管鳞癌预后的独立因素。
Objective： To investigate the expression pattern of stem cell factor HIWI and the significance of this pattern in the diag- nosis and prognosis of human esophageal squamous cell carcinoma （ESCC）. Methods： The expression of HIWI in 153 esophageal squa- mous cell carcinoma specimens was characterized using immunohistochemistry. The correlations between the clinicopathological fea- tures and the expression of HIWI in esophageal squamous cancer were analyzed using crosstabs analysis. The survival curve regarding the expression of HIWI was analyzed using the Kaplan-Meier method. Results： HIWI immunoreactivity was observed in both the nu- cleus and cytoplasm of the ESCC. The expression level of HIWI in the cytoplasm was significantly associated with the histological grade （P=0.011, X2=9.075） and the T stage （P=0,035, ）X2=8.589）. Patients with high HIWI expression tumors had poorer overall survival than those with low cytoplasmic expression tumors （P 〈 0.001, X2=17.05）. Conclusion： Higher cytoplasmic expression levels of stem cell factor HIWI in esophageal squamous carcinoma were significantly associated with higher histological grade and T stage, as well as poorer overall survival of the patients, making HIWI in cytoplasm an independent prognostic factor for survival.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第19期1401-1405,共5页
Chinese Journal of Clinical Oncology
关键词
食管鳞癌HIWI免疫组织化学预后
Esophageal squamous cell carcinoma
HIWI
Immunohistochemistry
Prognosis
