摘要
目的:用HPLC同时测定附子中盐酸多巴胺和去甲猪毛菜碱的含量。方法:采用Agilent Eclipse C18柱(4.6 mm×250 mm,5μm),流动相为0.1%磷酸水溶液,流速1 mL.min-1,柱温35℃,检测波长280 nm。结果:盐酸多巴胺和去甲猪毛菜碱色谱峰与其他色谱峰分离良好,样品中盐酸多巴胺和去甲猪毛菜碱色谱峰的DAD光谱与对照品一致,进样量在0.00521～0.521μg和0.005 24～0.524μg线性关系良好,平均回收率分别为100.6%和96.5%,RSD分别为2.0%和1.0%(n=6)。结论:实验含量测定方法准确、快速,为附子质量控制提供参考方法。
Objective：To establish a HPLC method for simultaneous determination of dopamine hydrochloride and salsolinol in Radix Aconite Lateralis.Method：The separation was carried on an Agilent Eclipse C18 column（4.6 mm×250 mm,5 μm）with 0.1% H3PO4 as mobile phase at a flow rate of 1 mL · min-1.The column temperature was set at 35 ℃ and the detector wavelength was at 280 nm.Result：Dopamine hydrochloride and salsolinol were all well separated from other substances and their UV spectrums were essentially the same to the standards.The liner ranges of dopamine hydrochloride and salsolinol were 0.005 21-0.521 μg,0.005 24-0.524 μg.The average recoveries were 100.6% and 96.5%;RSD were 2.0% and 1.0%（n=6）.Conclusion：This method is rapid and accurate and is suitable for quality control of Radix Aconite Lateralis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第19期70-73,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划(973计划)课题(2009CB522804)
国家科技支撑计划(2011BAI13B05)
