摘要
目的:了解乌头碱的水解转化规律。方法:将乌头碱单体置纯水中,密封加热8,12,16,20,24 h,水解液碱化萃取,采用HPLC-MS分析主要水解产物;进一步采用中性氧化铝柱色谱分离,得到主要水解产物,并鉴定结构。结果:经HPLC-MS分析,鉴定主要水解成分峰为苯甲酰乌头原碱和乌头原碱。水解20 h,苯甲酰乌头原碱的转化率最高。结论:双酯型的乌头碱可水解转化为单酯型乌头碱和乌头原碱;在控制条件下,苯甲酰乌头原碱为主要水解产物。
Objective：To find out hydrolysis regularity of aconitine.Method： Aconitine was air-tighted and hydrolyzed in water for 8,12,16,20,24 h.The hydrolysis products were analyzed by HPLC-MS.A major hydrolysis product was isolated by alumina oxide column chromatography,and identified by ^1H and ^13C-NMR spectra.Result： HPLC-MS analysis shows that the major hydrolysis products are benzoylaconine and aconine.The hydrolysis can mostly be completed within 20 hours.Conclution： Diester aconitum alkaloid can be changed to monoester aconitum and aconine alkaloids.Under the controled condition benzoylaconine is a major hydrolysis products.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第20期2143-2145,共3页
China Journal of Chinese Materia Medica
