摘要
目的:优化吴茱萸药材HPLC含量测定方法。方法:流动相[乙腈-四氢呋喃(25∶15)]-0.02%磷酸水溶液(35∶65),流速1 mL.min-1,检测波长220 nm。结果:柠檬苦素、吴茱萸碱、吴茱萸次碱色谱峰与其它色谱峰分离良好,样品中柠檬苦素、吴茱萸碱、吴茱萸次碱色谱峰的DAD光谱与对照品基本一致,进样量在0.196 8～3.936,0.153 6～3.072,0.097 4～1.948μg线性关系良好,平均回收率分别为97.8%,100.7%,98.4%,RSD分别为1.7%,1.3%,1.1%(n=6)。结论:建立的吴茱萸药材HPLC含量测定方法,测定结果准确,可信。
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [ acetonitrile-tetrahydrofuran（25： 15） ] -0. 02% H3PO4 （35： 65）. The detection wavelength was 220 nm and the flow rate was 1.0 mL min-1 . Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0. 196 8-3.936,0. 153 6-3. 072, 0. 097 4-1. 948 μg. The average recoveries were 97. 8% ,100. 7% and 98.4%. RSD were 1.7% ,1.3% and 1.1% （n =6）. The method of this article is accurate,reproducible and can be used to enhance the quality control of E. rutaecarpa.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第4期478-480,共3页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2009CB522804)
