摘要
目的:优选秋鼠曲草花中主要成分的提取工艺。方法:以槲皮素和芹菜素含量为综合评价指标,采用HPLC测定指标成分含量,采用单因素试验考察提取方式、料液比、提取次数及乙醇体积分数;选取提取次数、提取时间、料液比及乙醇体积分数为考察因素,正交设计试验优选提取工艺。结果:最佳提取工艺为超声提取,加入30倍量60%乙醇超声提取2次,每次40 min。结论:优选的提取工艺简单、稳定可行,可为秋鼠曲草花部分有效成分的定量分析提供可靠的提取方法。
Objective：To optimize extraction process of main components from flower of Gnaphalium hypoleucum.Method：With the contents of quercetin and apigenin as comprehensive evaluation index,which were determined by HPLC.Extraction method,ratio of solid-liquid,extraction times and the concentration of ethanol were investigated by single factor test;orthogonal design test was used to optimize extraction technology with extraction time,extraction times,ratio of solid-liquid and the concentration of ethanol.Result：Optimum extraction conditions were as follows：ultrasonic extracted 2 times with 30 times the amount of 60% ethanol,40 min per time.Conclusion：This optimized process was simple,stable and feasible.It could provide reliable extraction method for quantitative analysis of main components from flower of G.hypoleucum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第19期53-56,共4页
Chinese Journal of Experimental Traditional Medical Formulae
