摘要
为了研究葡萄籽原花青素提取物(Grape Seed Proanthocyanidin Extracts,GSPE)对糖尿病小鼠的降血糖作用及其机制,本文中采用腹腔注射四氧嘧啶(ALX)构建糖尿病动物模型。造模成功后,将糖尿病小鼠分为模型对照组、格列本脲处理组、葡萄籽原花青素提取物低、中、高三个剂量(50、100、150 mg/kg)处理组,另设正常对照组。连续灌胃给药21天,每天一次,以糖尿病小鼠的体重、血清丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性胰岛素水平、空腹血糖值和糖耐量为测定指标,研究不同剂量葡萄籽原花青素提取物对糖尿病小鼠的降血糖作用及机制。结果显示原花青素高剂量组能促进体重增长,显著降低糖尿病小鼠的血糖水平和糖耐量(P<0.01);同时降低糖尿病小鼠血清MDA水平,提高其SOD活性。通过本实验研究可以看出葡萄籽原花青素提取物具有较强的降血糖作用,降糖机制可能与其提高胰岛素水平和抗氧化能力有关。
To investigate hypoglycemic effect and mechanism of grape seed proanthocyanidin extracts（GSPE） on diabetic mice,the diabetic model were induced by peritoneal injection of alloxan.Then the diabetic mice were divided into model control group,glibenclamide treated group,three GSPE treated groups of different dose（50,100,150 mg/kg）,and normal control group was another one.The drugs were chronically administrated to diabetic mice once a day for 21 days.Body weights,MDA levels,SOD activity,insulin levels in serum,fasting blood glucose and glucose tolerance were determined as main indexes.The hypoglycemic effect and mechanism of GSPE in different dose were studied.The results showed that body weights of diabetic mice increased,fasting blood glucose and glucose tolerance levels of diabetic mice in GSPE group of high dose significantly decreased（P0.01）;further more MDA levels of diabetic mice decreased,and SOD activities were increased.We concluded that GSPE could significantly decrease blood glucose level,and the mechanism might be related to increase of insulin level and antioxidant activity of diabetic mice.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第9期1191-1195,共5页
Natural Product Research and Development
基金
黑龙江省基金项目(QC2011C126)
黑龙江八一农垦大学博士启动基金项目(校启B-32)
关键词
葡萄籽原花青素提取物
四氧嘧啶
糖耐量
降血糖机制
grape seed proanthocyanidin extracts
alloxan
glucose tolerance
hypoglycemic mechanism
