摘要
旨在研究松树皮原花青素提取物对糖尿病小鼠血糖的影响,并探讨其作用机制。通过高脂饲喂结合腹腔注射链脲佐菌素(STZ)造糖尿病小鼠模型,将造模成功的小鼠随机分为模型组,格列本脲组(250 mg/kg),松树皮原花青素高剂量组(400 mg/kg)、中剂量组(200 mg/kg)、低剂量组(100 mg/kg),连续灌胃8周后通过断尾取血测定糖尿病小鼠空腹血糖;摘眼球取血,分离血清,测定血脂、血清胰岛素(INS)含量、白细胞介素6(IL-6)含量、C反应蛋白(CRP)含量;用苏木素-伊红(HE)染色处理胰脏组织切片,光镜观察病理学改变。实验结果显示,与模型组比较,受试药高、中剂量组能显著降低STZ所致糖尿病小鼠的血糖、TG、TC含量,增加INS含量,显著降低IL-6和CRP含量(P<0.05);糖尿病小鼠胰脏病变组织得到修复。实验结果表明,松树皮原花青素提取物对糖尿病小鼠有明显的降低血糖、改善血脂水平的作用,其作用机制可能与其能修复胰岛受损β细胞,提升血清INS水平,降低炎症因子含量有关。
To study the effects of pine bark procyanidins extract on blood glucosein diabetic mice, to explore the related mechanism. Diabetic mice model was made by high fat diet combined with intraperitoneal injection of STZ. Diabetic mouse were randomly divided into model group, Glibenclamide group （250 mg/kg）, pine bark procyanidins extract high-dose group （400 mg/kg）, middle-dose group （200 mg/kg） and low-dose group （100 mg/kg）. Fasting blood glucose was measured after continuous intragastric administration 8 weeks. Blood samples were taken out from the tail of diabetic mice to measure fasting blood glucose;Then blood samples were taken out from the eyeball to measure IL-6 content, CRP content and blood lipid level. Pine bark procyanidins extract high and middle-dose group can significantly lower the blood glucose level in STZ induced diabetic mice （P〈0.05）.Serum TG and TC content in pine bark procyanidins extract high and middle-dose group werelower than the model group significantly（P〈0.05）.INS content in pine bark procyanidins extract high-dose group was higher than the model group significantly（P〈0.05）.Serum IL-6、CRP content in pine bark procyanidins extrac-thigh and middle-dose group were lower than the model group significantly. Diabetic mice pancreatic tissue were repaired. The pine bark procyanidins extract had a significant effects on reducing blood glucose and im-proving blood lipid level in STZ induced diabetic mice. The mechanism might be related to repairing pancreatic tissue, improving the INS and reducing the content of inflammatory factors in diabetic mice.
作者
王俊
任美萍
胡博
王芳
WANG Jun REN Mei-ping HU Bo WANG Fang(School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, China Research Center for Drug and Functional Food, Southwest Medical University, Luzhou 646000, Sichuan, China)
出处
《食品研究与开发》
CAS
北大核心
2017年第7期164-167,共4页
Food Research and Development
基金
四川省教育厅资助项目(14ZB0154)
关键词
松树皮原花青素提取物
糖尿病
血糖
血脂
pine bark procyanidins extract
diabetes
blood glucose
blood lipid
