摘要
目的:通过康艾注射液和苦参素对不同肿瘤细胞增殖抑制作用的对比研究,考察复方对抑瘤作用的影响。方法:(1)MTT法分别测定苦参素注射液、康艾注射液对体外培养的不同肿瘤细胞HCT-8、K-562、HO-8910、SGC-7901的抑率,求算IC50;(2)比较苦参素注射液和康艾注射液对不同瘤株的抑制率,考察不同肿瘤细胞对药物的敏感性差异;(3)比较苦参素注射液和康艾注射液对同一肿瘤细胞的抑制率,考察复方和单方抑瘤活性的差异。结果:(1)苦参素注射液和康艾注射液对HCT-8、K-562、HO-8910、SGC-7901的抑瘤率均随浓度的增高而增强,浓度与抑瘤率呈正相关,其相关系数r分别为:0.935、0.933、0.942、0.952和0.947、0.915、0.953、0.964;(2)苦参素注射液和康艾注射液对HCT-8、K-562、HO-8910、SGC-7901的IC50分别为:1012.03、719.28、681.64、1 034.15 mg.L-1和905.99、764.10、686.27、995.52 mg.L-1,苦参素注射液和康艾注射液对HO-8910细胞相对较为敏感;(3)相同浓度的苦参素注射液和康艾注射液体外对肿瘤细胞增殖的抑制作用无显著性差异(P>0.05),结果提示氧化苦参碱为康艾注射液中直接抑制肿瘤细胞增殖的主要有效成分。结论:康艾注射液有较好的抑瘤作用,其主要的直接抑瘤成分为苦参素,即氧化苦参碱。
OBJECTIVE： To investigate the inhibitory effects of the compound Kangai injection vs. Kushensu injection on different tumor cells. METHODS： The inhibitory effects of oxymatrine and Kangai injection on different tumor cells K-562, H0-8910, HCT-8 and SGC-7901 cultured in vitro were measured by MTr assay, and the ICs0 was obtained; the tumor-inhibition ratio of Kangai injection vs. Kushensu injection on different tumor cells were compared and the drug sensitivity of different tumor cells was compared as well; the inhibition ratio of Kangai injection vs. Kushensu injection on the same kind of tumor cells were measured ; the inhibition ratio of Kangai injection and Kushensu injection either alone or in combination on different tumor cells was investigated. RESULTS： The inhibitory effects of Kangai injection and Kushensu injection on different tumor cells increased dose-dependently, with the concentration strongly correlated to the inhibition ratio （correlation coefficient R of the two were 0. 935, 0. 933, 0. 942 and 0. 952, respeetilvey vs. 0. 947, 0. 915, 0. 953 and 0. 964, respectively. The IC50 of Kushensu and Kangai on HCT-8, K-562, H0-8910 and SGC-7901 were 1012. 03, 719. 28, 681.64, 1034. 15 mg. L-＇ respectively vs. 905.99, 764. 10, 686. 27 and 995. 52 mg. L -＇, respectively at the same concentration showed proliferation in vivo （ P 〉 0. 05 ） , indicating their good sensitivity to H0-8910. Kushenin injection and Kangai injection no statistically significant differences in terms of their inhibition effects on tumor cell , and the results indicated that oxymatrin in Kangai injection was the major active component with direct inhibitory effect on tumor cell proliferation. CONCLUSION： Kangai injection showed remarkable inhibitory effects on tumor cell proliferation and in which oxymatrine is the main tumor-inhibition ingredient.
出处
《中国医院用药评价与分析》
2012年第8期712-715,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
