摘要
目的建立HPLC同时测定菟丝子不同炮制品中绿原酸、金丝桃苷、槲皮素、山柰酚和异鼠李素的含量。方法色谱条件:Kromasil ODS-1色谱柱(250 mm×4.6 mm,5μm),甲醇-0.4%磷酸水溶液为流动相,梯度洗脱,流速为1 mL/min,柱温为30℃,检测波长为360 nm。结果 5种活性成分均达到基线分离,线性关系良好(r≥0.999 7);平均加样回收率为98.4%～100.7%,RSD≤2.4%(n=6)。结论该方法灵敏、可靠,可用于菟丝子饮片的质量评价。
Objective To establish the method for quantitative analysis of chlorogenic acid, hyperoside, quercetin, kaempferol and isorhamnetin in the Cuscuta chinensis different pro- cessed products by HPLC. Methods The Kromasil C18 column （250 ram×4.6 mm,5 μm） was used with methanol-0.4% phosphoric acid as mobile phase. Under the condition of gradient elution, the flow rate was 1.0 mL/min, the column temperature was 30℃ and the detection wavelength was 360 nm. Results Baseline separation was obtained separately for the five kinds of active ingredients and there was a good linear relationship （r≥0.999 7）. The average recoveries were 98.4%-100.7% and RSD≤2.4%（n=6）. Conclusion The established method in this study is sensitive,reliable and available for the quality control of the different processed products of Cuscuta chinensis.
出处
《湖南中医药大学学报》
CAS
2012年第7期50-53,共4页
Journal of Hunan University of Chinese Medicine
关键词
菟丝子
绿原酸
金丝桃苷
槲皮素
HPLC
Cuscuta chinensis
chlorogenic acid
hyperoside
quercetin
HPLC
